Biotin Anti-VSV-G tag 抗体 (ab34774)
Key features and details
- Biotin Rabbit polyclonal to VSV-G tag
- Suitable for: ELISA, WB, ICC/IF
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-VSV-G tag antibody
VSV-G tag 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to VSV-G tag -
由来種
Rabbit -
標識
Biotin -
特異性
This affinity purified antibody is directed against VSV-G protein.
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アプリケーション
適用あり: ELISA, WB, ICC/IFmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide corresponding to VSV-G tag aa 501-511.
Sequence:YTDIEMNRLGK
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特記事項
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Donkey IgG molecule.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This affinity purified antibody is directed against VSV-G protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification followed by biotin conjugation, subsequent purification and exhaustive dialysis against the buffer. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34774の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | (1) |
1/10000 - 1/50000.
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| WB | (2) |
1/2000 - 1/10000. Predicted molecular weight: 57.5 kDa.
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| ICC/IF | (2) |
1/200 - 1/1000.
We recommend paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein. |
| 特記事項 |
|---|
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ELISA
1/10000 - 1/50000. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 57.5 kDa. |
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ICC/IF
1/200 - 1/1000. We recommend paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein. |
ターゲット情報
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関連性
Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding. -
別名
- Vesicular stomatitis virus glycoprotein tag antibody
- VSV epitope tag antibody
- VSV tag antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Biotin Anti-VSV-G tag antibody (ab34774)Image courtesy of an anonymous Abreview.ab34774 staining VSV-G tag in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.025% Triton-X in PBS and then blocked using 5% serum for 1 hour. Samples were then incubated with primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat polyclonal conjugated to Alexa Fluor® 488 (green) used undiluted.
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Western blot - Biotin Anti-VSV-G tag antibody (ab34774)Image courtesy of an anonymous Abreview.Both lanes are ab34774 at a 1/500 dilution plus 20µg of whole cell lysate prepared from HeLa cells transfected with VSV-G. A Streptavidin HRP conjugate was used at a 1/2000 dilution.
Primary antibody incubated for 16 hours at 4°C.Blocking step performed using 5% milk for 1 hour at 23°C. -
ELISA - Biotin Anti-VSV-G tag antibody (ab34774)This image is courtesy of an anonymous Abreviewab34774 detecting the VSV-G tag in HeLa cells expressing VSV-G by direct ELISA. Samples were blocked with 5% BSA for 1 hour at 23°C and incubated with the primary antibody (1/3000 in blocking buffer) for 16 hours at 4°C. An undiluted streptavidin-alkaline phosphatase conjugate polyclonal was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab34774 は 6 報の論文で使用されています。
- Kamps D et al. Optogenetic Tuning Reveals Rho Amplification-Dependent Dynamics of a Cell Contraction Signal Network. Cell Rep 33:108467 (2020). PubMed: 33264629
- Thompson A et al. Molecular Characterisation of Small Molecule Agonists Effect on the Human Glucagon Like Peptide-1 Receptor Internalisation. PLoS One 11:e0154229 (2016). PubMed: 27100083
- Thompson A & Kanamarlapudi V Distinct regions in the C-Terminus required for GLP-1R cell surface expression, activity and internalisation. Mol Cell Endocrinol 413:66-77 (2015). PubMed: 26116235
- Wan Z et al. Fangchinoline inhibits human immunodeficiency virus type 1 replication by interfering with gp160 proteolytic processing. PLoS One 7:e39225 (2012). WB . PubMed: 22720080
- Fukuhara T et al. Intracellular delivery of serum-derived hepatitis C virus. Microbes Infect 13:405-12 (2011). PubMed: 21262370
- Tani H et al. Replication-competent recombinant vesicular stomatitis virus encoding hepatitis C virus envelope proteins. J Virol 81:8601-12 (2007). WB . PubMed: 17553880