Biotin Anti-TSLP 抗体 (ab84247)
Key features and details
- Biotin Rabbit polyclonal to TSLP
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-TSLP antibody
TSLP 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to TSLP -
由来種
Rabbit -
標識
Biotin -
アプリケーション
適用あり: Sandwich ELISA, WBmore details -
種交差性
交差種: Human -
免疫原
Highly pure (>98%) recombinant Human TSLP.
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ポジティブ･コントロール
- WB: Recombinant Human TSLP protein (ab50020).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84247の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use a concentration of 0.25 - 1 µg/ml.
Use 100 μl/well antibody solution. Allows the detection of at least 0.2 – 0.4 ng/well of recombinant hTSLP. |
|
| WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 18 kDa.
The detection limit for recombinant hTSLP is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| 特記事項 |
|---|
|
Sandwich ELISA
Use a concentration of 0.25 - 1 µg/ml. Use 100 μl/well antibody solution. Allows the detection of at least 0.2 – 0.4 ng/well of recombinant hTSLP. |
|
WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 18 kDa. The detection limit for recombinant hTSLP is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
ターゲット情報
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機能
Cytokine that induces the release of T cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells. -
組織特異性
Expressed in a number of tissues including heart, liver and prostate. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 85480 Human
- Omim: 607003 Human
- SwissProt: Q969D9 Human
- Unigene: 389874 Human
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別名
- Thymic stromal lymphopoietin antibody
- Thymic stromal lymphopoietin protein TSLP antibody
- Tslp antibody
see all
画像
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Western blot - Biotin Anti-TSLP antibody (ab84247)
All lanes: Anti-TSLP antibody (ab84247) at 0.2 µg/ml
Lane 1: Marker
Unreduced Recombinant Human TSLP protein (ab50020):
Lane 2: 250 ng
Lane 3: 125 ng
Lane 4: 62.5 ng
Lane 5: 31.25 ng
Lane 6: 15.625 ng
Lane 7: 7.8 ng
Lane 8: 3.9 ng
Lane 9: 1.95 ng
Lane 10: 0.975 ng
Lane 11: 0.4875 ng
Lane 12: 0.24 ng
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Sandwich ELISA - Biotin Anti-TSLP antibody (ab84247)To detect human TSLP by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Rabbit polyclonal to TSLP (ab47943) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human TSLP.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab84247 は論文での使用が確認できていません。