Biotin Anti-Transferrin 抗体 (ab34673)
Key features and details
- Biotin Rabbit polyclonal to Transferrin
- Suitable for: WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-Transferrin antibody
Transferrin 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to Transferrin -
由来種
Rabbit -
標識
Biotin -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Transferrin from Human serum.
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特記事項
10-20 Biotin molecules per rabbit IgG molecule.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
精製度
Ion Exchange Chromatography -
特記事項（精製）
Anti-Transferrin Antibody, Biotin Conjugated, is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34673の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 77 kDa.
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| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 77 kDa. |
ターゲット情報
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機能
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. -
組織特異性
Expressed by the liver and secreted in plasma. -
関連疾患
Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. -
配列類似性
Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 7018 Human
- Omim: 190000 Human
- SwissProt: P02787 Human
- Unigene: 518267 Human
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別名
- Apotransferrin antibody
- Beta 1 metal binding globulin antibody
- Beta-1 metal-binding globulin antibody
see all
画像
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Western blot - Biotin Anti-Transferrin antibody (ab34673)
Multiplex western blot analysis of 35 µg of human serum with 350 ng of added GST. Green bands are labelling human transferrin, with ab34673 and a donkey anti-rabbit DyLight® 549 conjugate. Red bands are labelling trypsin, with ab7633 and a donkey anti-goat DyLight® 649 conjugate. Blue bands are labelling human IgG with a primary and a donkey anti-mouse DyLight® 488 conjugate. All primaries were diluted to 1/1,000 and incubated overnight at 4°C. All secondaries were diluted to 1/10,000 for 30 minutes at 4°C.
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Western blot - Biotin Anti-Transferrin antibody (ab34673)Biotin Anti-Transferrin antibody (ab34673) at 1/1000 dilution + Human depleted serum with Blocking buffer
Secondary
Peroxidase streptavidin conjugate at 1/40000 dilution
Performed under reducing conditions.
Predicted band size: 77 kDa -
Western blot - Biotin Anti-Transferrin antibody (ab34673)Multiplex western blot analysis of human serum. Green bands are labelling human transferrin, with ab34673 and a DyLight® 549 conjugate. Red bands are labelling trypsin, with ab7633 and a DyLight® 649 conjugate. Blue bands are labelling human IgG with a primary and a DyLight® 488 conjugate. All primaries were diluted to 1/1,000 and incubated overnight at 4°C. All secondaries were diluted to 1/10,000 for 1 hour at 4°C.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab34673 は論文での使用が確認できていません。