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Biotin Anti-TLR2 抗体 [TL2.1] (ab9101)

Submit a review Q&A (1)References (8)
Immunohistochemistry (Frozen sections) - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)
  • Flow Cytometry - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)

Key features and details

  • Biotin Mouse monoclonal [TL2.1] to TLR2
  • Suitable for: IHC-Fr, Flow Cyt, IHC-P
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

Carrier Free Unconjugated

製品の概要

  • 製品名

    Biotin Anti-TLR2 antibody [TL2.1]
    TLR2 一次抗体 製品一覧

  • 製品の詳細

    Biotin Mouse monoclonal [TL2.1] to TLR2

  • 由来種

    Mouse

  • 標識

    Biotin

  • アプリケーション

    適用あり: IHC-Fr, Flow Cyt, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant human TLR2 produced by the CHO/CD14 reporter cell line.

  • 特記事項

    Ab9101 is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human pheriperal blood mononuclear cells.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9101の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 
IHC-P
Use at an assay dependent concentration.
特記事項
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 
IHC-P
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.

  • 組織特異性

    Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.

  • 配列類似性

    Belongs to the Toll-like receptor family.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.

  • 翻訳後修飾

    Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD282 antibody
      • CD282 antigen antibody
      • TIL 4 antibody
      • TIL4 antibody
      • TLR 2 antibody
      • TLR2 antibody
      • TLR2_HUMAN antibody
      • Toll like receptor 2 antibody
      • Toll like receptor 2 precursor antibody
      • Toll-like receptor 2 antibody
      • Toll/interleukin 1 receptor like 4 antibody
      • Toll/interleukin 1 receptor like protein 4 antibody
      • Toll/interleukin receptor like protein 4 antibody
      • Toll/interleukin-1 receptor-like protein 4 antibody
      see all

    画像

    • Immunohistochemistry (Frozen sections) - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)
      Immunohistochemistry (Frozen sections) - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)

      Immunohistochemistry analysis of human brain frozen tissue tissue labelling TLR2 with ab9101

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)

      Immunohistochemical analysis of human TLR2 in paraffin-embedded human spleen tissue.

    • Flow Cytometry - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)
      Flow Cytometry - Biotin Anti-TLR2 antibody [TL2.1] (ab9101)
      TLR2 staining on human peripheral blood mononuclear cells by using ab9101. Green: CD3+-cells; Red: CD14+ cells.

    参考文献 (8)

    ab9101 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9101 は 8 報の論文で使用されています。

    • Bridi M  et al. Transcriptional corepressor SIN3A regulates hippocampal synaptic plasticity via Homer1/mGluR5 signaling. JCI Insight 5:N/A (2020). PubMed: 32069266
    • Zhao Q  et al. Xiaozhang Tie Improves Intestinal Motility in Rats With Cirrhotic Ascites by Regulating the Stem Cell Factor/c-kit Pathway in Interstitial Cells of Cajal. Front Pharmacol 11:1 (2020). PubMed: 32116689
    • Steinberger J  et al. Tracing MYC Expression for Small Molecule Discovery. Cell Chem Biol 26:699-710.e6 (2019). PubMed: 30880156
    • Howard L  et al. CD40 forward signalling is a physiological regulator of early sensory axon growth. Development 146:N/A (2019). PubMed: 31488565
    • Zhang P  et al. Toll-Like Receptors 2 and 4 Predict New-Onset Atrial Fibrillation in Acute Myocardial Infarction Patients. Int Heart J 59:64-70 (2018). PubMed: 29375116
    • Flo TH  et al. Differential expression of Toll-like receptor 2 in human cells. J Leukoc Biol 69:474-81 (2001). PubMed: 11261796
    • Wang JE  et al. Involvement of CD14 and toll-like receptors in activation of human monocytes by Aspergillus fumigatus hyphae. Infect Immun 69:2402-6 (2001). PubMed: 11254600
    • Flo TH  et al. Human toll-like receptor 2 mediates monocyte activation by Listeria monocytogenes, but not by group B streptococci or lipopolysaccharide. J Immunol 164:2064-9 (2000). PubMed: 10657659

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    I am interested in your TLR2 antibody for Western blotting. Can you give me some information about what target cells you used and what the conditions were for this assay.

    Read More
    Answer

    The following article describes the use of HM2064 (monoclonal antibody against human TLR-2, clone TL2.1): Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NK-kappa B activation. E.Faure et al. J. Immunol 2001, 166 p. 2018.

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