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AB314404

Biotin Anti-Tau (phospho T205) 抗体 [EPR23505-13]

Biotin Anti-Tau (phospho T205) antibody [EPR23505-13]

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Rabbit Recombinant Monoclonal TAU phospho T205 antibody - conjugated to Biotin. Suitable for IHC-P, I-ELISA, WB and reacts with Mouse, Human, Rat, Synthetic peptide samples.

別名を表示する

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, ptau, p tau, phospho tau, ptau 205

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Tau (phospho T205) antibody [EPR23505-13] (AB314404)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Tau (phospho T205) antibody [EPR23505-13] (AB314404)

Negative staining of Tau (phospho T205) in a section of formalin-fixed paraffin-embedded mouse liver. Performed on a Leica BOND™ system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab314404 at 5 µg /ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Tau (phospho T205) antibody [EPR23505-13] (AB314404)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Tau (phospho T205) antibody [EPR23505-13] (AB314404)

IHC image of Tau (phospho T205) staining in a section of formalin-fixed paraffin-embedded mouse cerebrum. Performed on a Leica BOND™ system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab314404 at 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Positive staining on mouse cerebrum without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The inset secondary-only control image is taken from an identical assay without primary antibody. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Indirect ELISA - Biotin Anti-Tau (phospho T205) antibody [EPR23505-13] (AB314404)
  • I-ELISA

Lab

Indirect ELISA - Biotin Anti-Tau (phospho T205) antibody [EPR23505-13] (AB314404)

Indirect ELISA showing primary antibody ab314404 binding to Tau peptides. Plates were coated with Tau (T205) phospho peptide (positive control), Tau unmodified peptide (negative control) and Tau (T181) phospho peptide (negative control) at 100 ng/ml. Binding of ab314404 was assessed in a serial dilution range 0.016- 100 ng/mL (a 3-fold serial dilution). Binding was detected using horseradish peroxidase Streptavidin at 1 g/ml final concentration. IC-50 was determined to be 6.714 ng/ml.

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    Anti-Tau (phospho T205) antibody [EPR23505-13]

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    Anti-Tau (phospho T205) antibody [EPR23505-13] - BSA and Azide free

  • 660 APC

    APC Anti-Tau (phospho T205) antibody [EPR23505-13]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Tau (phospho T205) antibody [EPR23505-13]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Tau (phospho T205) antibody [EPR23505-13]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Tau (phospho T205) antibody [EPR23505-13]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Tau (phospho T205) antibody [EPR23505-13]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Tau (phospho T205) antibody [EPR23505-13]

  • 578 PE

    PE Anti-Tau (phospho T205) antibody [EPR23505-13]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR23505-13

アイソタイプ

IgG

標識

Biotin

励起波長/蛍光波長
キャリアフリー

No

交差種

Mouse, Human, Mouse, Rat

アプリケーション

WB, IHC-P, I-ELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Proteins & Peptides

AB316441

Recombinant Human Tau Protein Standard (His tag)

Sandwich ELISA - Recombinant Human Tau Protein Standard (His tag) (AB316441)

  • 0
  • 0
Primary Antibodies

AB76128

Anti-Tau antibody [EP2456Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Western blot - Anti-Tau antibody [EP2456Y] (AB76128)

  • 5
  • 1
Primary Antibodies

AB309970

Alexa Fluor® 647 Anti-Tau (phospho T181) antibody [EPR23506-107]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Tau (phospho T181) antibody [EPR23506-107] (AB309970)

  • 0
  • 0
Primary Antibodies

AB109390

Anti-Tau (phospho S396) antibody [EPR2731]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (AB109390)

  • 4
  • 14
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Synthetic peptide": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "0.016-100 ng/mL", "IELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
See full target information MAPT pT205
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Abcam product promise

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