Biotin Anti-sRANKL 抗体 (ab83145)
Key features and details
- Biotin Rabbit polyclonal to sRANKL
- Suitable for: Sandwich ELISA, WB
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-sRANKL antibody
sRANKL 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to sRANKL -
由来種
Rabbit -
標識
Biotin -
アプリケーション
適用あり: Sandwich ELISA, WBmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Highly pure (>98%) recombinant human sRANKL.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Centrifuge vial prior to opening. Reconstitute in 50 µl sterile PBS containing 0.1% BSA to give a concentration of 1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab83145 is sterile filtered. ab83145 was purified by affinity chromatography and then biotinylated. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab83145の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use a concentration of 0.25 - 1 µg/ml.
Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human sRANKL. |
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| WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 28 kDa.
The detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non reducing conditions. |
| 特記事項 |
|---|
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Sandwich ELISA
Use a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human sRANKL. |
|
WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 28 kDa. The detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non reducing conditions. |
ターゲット情報
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機能
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. -
組織特異性
Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. -
関連疾患
Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. -
配列類似性
Belongs to the tumor necrosis factor family. -
翻訳後修飾
The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17. -
細胞内局在
Cytoplasm; Secreted and Cell membrane. - Information by UniProt
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別名
- CD254 antibody
- hRANKL2 antibody
- ODF antibody
see all
画像
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Western blot - Biotin Anti-sRANKL antibody (ab83145)
To detect human sRANKL by Western Blot analysis ab83145 can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human sRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is non-reducing conditions; Human recombinant sRANKL protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 human recombinant sRANKL protein.
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Sandwich ELISA - Biotin Anti-sRANKL antibody (ab83145)To detect human sRANKL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83145 is required. This biotinylated polyclonal antibody, in conjunction with an anti-RANKL (ab9957) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human sRANKL.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab83145 は論文での使用が確認できていません。