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AB83135

Biotin Anti-RANTES 抗体

Biotin Anti-RANTES antibody

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Rabbit Polyclonal RANTES antibody - conjugated to Biotin. Carrier free. Suitable for WB, sELISA and reacts with Recombinant full length protein - Mouse samples.

別名を表示する

Scya5, Ccl5, C-C motif chemokine 5, MuRantes, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES

2 Images
Sandwich ELISA - Biotin Anti-RANTES antibody (AB83135)
  • sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-RANTES antibody (AB83135)

To detect mouse RANTES by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83135 is required. This biotinylated polyclonal antibody, in conjunction with a appropriate anti-RANTES antibody as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mouse RANTES.

Western blot - Biotin Anti-RANTES antibody (AB83135)
  • WB

Supplier Data

Western blot - Biotin Anti-RANTES antibody (AB83135)

To detect murine RANTES by Western Blot analysis ab83135 can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant murine RANTES is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Image shown is reducing conditions; Murine recombinant RANTES protein at 250 - 0.24 ng/ml.

Lane 1 is MW marker, lanes 2-12 murine recombinant RANTES protein.

All lanes:

Western blot - Biotin Anti-RANTES antibody (ab83135)

Predicted band size: 10 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

標識

Biotin

励起波長/蛍光波長
キャリアフリー

Yes

アプリケーション

WB, sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.10000-0.20000 µg/mL", "WB-species-notes": "<p>The detection limit for recombinant murine RANTES is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.</p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.25000-1 µg/mL", "sELISA-species-notes": "<p>Allows the detection of at least 0.2 - 0.4 ng/well of recombinant murine RANTES.</p>" } } }
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こちらの製品も推奨する理由

この製品は、同様の実験や関連する研究で広く使用されていることから推奨させていただいております。

お客様の実験に適するかご確認いただくため、必ず製品データシートをご確認いただくことをお勧めいたします。ご不明点等ございましたら、テクニカルサポートチームまでお問い合わせください。

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出荷温度及び保存条件

製品の状態
Lyophilized
再構成
Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
精製方法
Affinity purification Protein A
精製に関する特記事項
ab83135 is sterile filtered.
バッファー組成
Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
See full target information Ccl5
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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