Biotin Anti-Protein A 抗体 (ab18598)
Key features and details
- Biotin Chicken polyclonal to Protein A
- Suitable for: WB, ELISA
- Reacts with: Staphylococcus aureus
- Conjugation: Biotin
- Isotype: IgY
製品の概要
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製品名
Biotin Anti-Protein A antibody
Protein A 一次抗体 製品一覧 -
製品の詳細
Biotin Chicken polyclonal to Protein A -
由来種
Chicken -
標識
Biotin -
アプリケーション
適用あり: WB, ELISAmore details -
種交差性
交差種: Staphylococcus aureus -
免疫原
Recombinant full length protein corresponding to Staphylococcus aureus Protein A.
Database link: P02976 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
After multiple immunizations in Freund’s adjuvant, eggs were collected and delipidated. Antibodies were immunoaffinity purified off protein A agarose and conjugated with biotin. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgY -
研究分野
関連製品
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18598の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/5000.
|
|
ELISA |
1/10000 - 1/50000.
|
特記事項 |
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WB
1/1000 - 1/5000. |
ELISA
1/10000 - 1/50000. |
ターゲット情報
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関連性
Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. -
細胞内局在
Secreted; cell wall. -
参照データベース
- Entrez Gene: 3919448 Staphylococcus aureus
- SwissProt: B3GGB2 Staphylococcus aureus
- SwissProt: P02976 Staphylococcus aureus
- SwissProt: P38507 Staphylococcus aureus
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別名
- gG-binding protein A antibody
- Immunoglobulin G binding protein A antibody
- SPA antibody
- Staphylococcal protein A antibody
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab18598 は 2 報の論文で使用されています。
- Abuaita BH et al. Mitochondria-Derived Vesicles Deliver Antimicrobial Reactive Oxygen Species to Control Phagosome-Localized Staphylococcus aureus. Cell Host Microbe 24:625-636.e5 (2018). PubMed: 30449314
- Gilbert FB et al. Differential response of bovine mammary epithelial cells to Staphylococcus aureus or Escherichia coli agonists of the innate immune system. Vet Res 44:40 (2013). WB ; Staphylococcus aureus . PubMed: 23758654