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AB18598

Biotin Anti-Protein A 抗体

Biotin Anti-Protein A antibody

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(2 Publications)

Anti-Protein A antibody - Biotin conjugated (ab18598) is a chicken polyclonal antibody detecting Protein A in Western Blot, ELISA. Suitable for Staphylococcus aureus.

- Trusted since 2005

別名を表示する

SAOUHSC_00069, spa, Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SpA

Key facts

宿主種

Chicken

クローン性

Polyclonal

アイソタイプ

IgY

標識

Biotin

励起波長/蛍光波長
キャリアフリー

No

交差種

Staphylococcus aureus

アプリケーション

WB, ELISA

applications

免疫原

Recombinant Full Length Protein corresponding to Staphylococcus aureus subsp. aureus NCTC 8325 spa.

P02976

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Staphylococcus aureus": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

製品の詳細

What is this antibody validated in?
Biotin Anti-Protein A antibody (ab18598) is a chicken polyclonal antibody and is validated for use in Western Blot (WB), ELISA in Staphylococcus aureus samples.

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
After multiple immunizations in Freund’s adjuvant, eggs were collected and delipidated. Antibodies were immunoaffinity purified off protein A agarose and conjugated with biotin.
バッファー組成
pH: 7.2 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Avoid freeze / thaw cycle

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Protein A also known as Staphylococcus aureus Protein A is a bacterial protein with a molecular weight of approximately 42 kDa. It is found in the cell wall of the bacterium Staphylococcus aureus. This protein binds strongly to immunoglobulins especially the Fc region of IgG making it a useful tool in biochemical research. This binding property is used to isolate antibodies and also in various immunoassays such as Protein A ELISA kits.
Biological function summary

Protein A from Staphylococcus aureus plays a role in the evasion of host immune responses. It helps the bacterium avoid detection by immune cells aiding in its pathogenicity. Protein A does not form part of any large complex but interacts directly with IgG molecules. This ability to bind antibodies is leveraged in the laboratory to purify antibody-antigen complexes which can be critical for research and diagnostic applications.

Pathways

Its role is more mechanical than part of any classic biochemical pathways but it contributes to evasion of the host immune pathway. It has connections with immune signaling proteins such as the Fc receptors found on immune cells. The interception and binding of antibodies by Protein A can modulate the normal immune signaling pathways thereby impacting immune surveillance and response.

Protein A's interaction with the immune system links it to conditions involving Staphylococcus aureus infections such as pneumonia and skin infections. Its ability to interfere with antibody function contributes to the virulence of Staphylococcus aureus and complicates the immune response. Its connection to Fc receptor proteins illustrates its potential role in evading immune reactions during the infectious disease process presenting challenges in treatment and diagnosis.

製品プロトコール

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ターゲットの情報

Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (PubMed : 10805799, PubMed : 2938951, PubMed : 4163007). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).
See full target information spa

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cell host & microbe 24:625-636.e5 PubMed30449314

2018

Mitochondria-Derived Vesicles Deliver Antimicrobial Reactive Oxygen Species to Control Phagosome-Localized Staphylococcus aureus.

Applications

Unspecified application

Species

Unspecified reactive species

Basel H Abuaita,Tracey L Schultz,Mary X O'Riordan

Veterinary research 44:40 PubMed23758654

2013

Differential response of bovine mammary epithelial cells to Staphylococcus aureus or Escherichia coli agonists of the innate immune system.

Applications

WB

Species

Staphylococcus aureus

Florence B Gilbert,Patricia Cunha,Kirsty Jensen,Elizabeth J Glass,Gilles Foucras,Christèle Robert-Granié,Rachel Rupp,Pascal Rainard
View all publications

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