AB53449

Biotin Anti-Polyethylene glycol 抗体 [PEG-B-47b]

Name: Biotin Anti-Polyethylene glycol 抗体 [PEG-B-47b] (ab53449) | Abcam
Brand: Abcam Limited
SKU: AB53449
Price: 83000 JPY
Availability: InStock
Rating: 5 (1 reviews)

Biotin Anti-Polyethylene glycol antibody [PEG-B-47b]

RabMAb
Recombinant
詳細を見る

(1 Review)

(15 Publications)

Anti-Polyethylene glycol antibody [PEG-B-47b] - Biotin conjugated (ab53449) is a rabbit recombinant monoclonal antibody detecting Polyethylene glycol in Western Blot, IHC-P, ELISA.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
- Trusted since 2007

別名を表示する

PEG

6 Images

ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)

ELISA

Unknown

ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)

Direct ELISA antigen dose-response curve using purified ab53449. Antigen concentration of 0-500 ng/mL. A peroxidase-conjugated steptavidin (1/1000) was used.

Biotin conjugate (ab53449) available for this clone.

ELISA

Unknown

ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)

ELISA assay using 5 μg/mL Anti-AFP RabMAb as the capture antibody and 0.5 μg/mL Anti-PEG-47-Biotin RabMAb (unpurified ab53449) as the detection antibody. HRP-avidin (1/3000) used for anti-PEG-47 detection. This ELISA assay could detect YCA1017 peptide-PEG antigen at a concentration as low as 0.9 ng/mL.

sELISA

Unknown

Sandwich ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)

Sandwich ELISA antigen dose-response curve using purified ab53449.

Capture antibody - Mouse anti-IFN alpha 2b (4000 ng/ml).

Antigens - PEG-IFN alpha 2b (0-1000 ng/ml).

Detection - ab53449 (500 ng) anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) followed by peroxidase-conjugated streptravidin (1/1000).

ELISA

Unknown

ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)

Direct ELISA assay using 63 ng/mL of antigen (GCSF-PEG) to coat the plate. HRP-avidin (1/3000) was used for the detection of varying amounts of anti-PEG-47-Biotin (unpurified ab53449).

Lab

Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/50000 dilution

All lanes:

BSA-PEG5K at 0.02 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 2 kDa

Observed band size: 280 kDa

false

Unknown

Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)

Lane 1:

Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/5000 dilution

Lane 2:

Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/10000 dilution

Lane 3:

Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/20000 dilution

All lanes:

BSA-PEG

Predicted band size: 2 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

PEG-B-47b

アイソタイプ

IgG

標識

Biotin

励起波長/蛍光波長

キャリアフリー

アプリケーション

IHC-P, WB, ELISA, sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

ab53449 does not cross react with Tween20.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1 µg/mL", "ELISA-species-notes": "Coating plates for ELISA: 0 to 500 ng/ml.For unpurified use at 0.5μg/ml, coating plates for ELISA: 5 to 10 ug/ml.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000", "WB-species-notes": "For unpurified use at 1/5000 - 1/20000.", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "For unpurified use at 1/50 - 1/100. Images of positive IHC with this monoclonal antibody, clone PEG-B-47b, are on the <a href='/products/primary-antibodies/polyethylene-glycol-antibody-peg-b-47-ab51257'>ab51257</a> datasheet. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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製品の詳細

Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ELISA, IHC-P, WB, sELISA.

Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) has been cited over 15 times in peer reviewed journals and is trusted by the scientific community.

Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) is sold in a 100 ug selling size.

Conjugation-ready, carrier free format available for antibody clone (PEG-B-47b) - ab271853.

The smallest PEG molecule which our Polyethylene glycol antibody (ab53449) can detect is PEG750. Based on customer feedback, the largest PEG molecule detected is PEG 40K. Additionally, ab53449 detects both linear and branched PEG based on our in-house ELISA testing.

Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. Our anti-mPEG antibody [PEG-B-47b] reacts to the methoxy group of Polyethylene glycol (PEG). There is no methoxy group in Tween 20, which should not interfere with the function of this antibody.

If you have any questions on our PEG products - please visit our Polyethylene glycol (PEG) FAQs page.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Polyethylene glycol

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文献 (15)

Recent publications for all applications. Explore the full list and refine your search

Journal of colloid and interface science 610:766-774 PubMed34848062

2021

Screening of the binding affinity of serum proteins to lipid nanoparticles in a cell free environment.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Sebastiani,Marianna Yanez Arteta,Lennart Lindfors,Marité Cárdenas

ACS nano 15:6709-6722 PubMed33754708

2021

Apolipoprotein E Binding Drives Structural and Compositional Rearrangement of mRNA-Containing Lipid Nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Sebastiani,Marianna Yanez Arteta,Michael Lerche,Lionel Porcar,Christian Lang,Ryan A Bragg,Charles S Elmore,Venkata R Krishnamurthy,Robert A Russell,Tamim Darwish,Harald Pichler,Sarah Waldie,Martine Moulin,Michael Haertlein,V Trevor Forsyth,Lennart Lindfors,Marité Cárdenas

Transplantation 104:744-753 PubMed31929419

2020

Liposomal Delivery Improves the Efficacy of Prednisolone to Attenuate Renal Inflammation in a Mouse Model of Acute Renal Allograft Rejection.

Applications

Unspecified application

Species

Unspecified reactive species

Carla M A van Alem,Martina Schmidbauer,Song Rong,Katja Derlin,Jessica Schmitz,Jan H Bräsen,Anja Thorenz,Rongjun Chen,Jurjen M Ruben,Elizabeth M Winter,Maaike Schilperoort,Sander Kooijman,Reshma A Lalai,Josbert M Metselaar,Christian Klemann,Martin Meier,Cees van Kooten,Faikah Gueler,Joris I Rotmans

Stem cell reports 14:91-104 PubMed31839542

2019

Conformal Coating of Stem Cell-Derived Islets for β Cell Replacement in Type 1 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Aaron A Stock,Vita Manzoli,Teresa De Toni,Maria M Abreu,Yeh-Chuin Poh,Lillian Ye,Adam Roose,Felicia W Pagliuca,Chris Thanos,Camillo Ricordi,Alice A Tomei

Acta biomaterialia 97:461-473 PubMed31344512

2019

Computer-assisted engineering of programmed drug releasing multilayer nanomedicine via indomethacin-mediated ternary complex for therapy against a multidrug resistant tumor.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Che,Zhirui Liu,Dong Wang,Chunmei Xu,Chengyuan Zhang,Jin Meng,Jun Zheng,Hongfeng Yuan,Guanren Zhao,Xing Zhou

Nanomedicine : nanotechnology, biology, and medici 11:1039-46 PubMed25791806

2015

Prednisolone-containing liposomes accumulate in human atherosclerotic macrophages upon intravenous administration.

Applications

Unspecified application

Species

Unspecified reactive species

Fleur M van der Valk,Diederik F van Wijk,Mark E Lobatto,Hein J Verberne,Gert Storm,Martine C M Willems,Dink A Legemate,Aart J Nederveen,Claudia Calcagno,Venkatesh Mani,Sarayu Ramachandran,Maarten P M Paridaans,Maarten J Otten,Geesje M Dallinga-Thie,Zahi A Fayad,Max Nieuwdorp,Dominik M Schulte,Josbert M Metselaar,Willem J M Mulder,Erik S Stroes

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 15:88-100 PubMed25488654

2014

FR104, an antagonist anti-CD28 monovalent fab' antibody, prevents alloimmunization and allows calcineurin inhibitor minimization in nonhuman primate renal allograft.

Applications

Unspecified application

Species

Unspecified reactive species

N Poirier,N Dilek,C Mary,S Ville,F Coulon,J Branchereau,X Tillou,V Charpy,S Pengam,V Nerriere-Daguin,J Hervouet,D Minault,S Le Bas-Bernardet,K Renaudin,B Vanhove,G Blancho

Journal of immunology (Baltimore, Md. : 1950) 191:4599-610 PubMed24081989

2013

A monovalent anti-human CD28 domain antibody antagonist: preclinical efficacy and safety.

Applications

ELISA

Species

Unspecified reactive species

Suzanne J Suchard,Patricia M Davis,Selena Kansal,Dawn K Stetsko,Ruth Brosius,James Tamura,Lumelle Schneeweis,James Bryson,Theodora Salcedo,Haiqing Wang,Zheng Yang,Catherine A Fleener,Olga Ignatovich,Christopher Plummer,Steven Grant,Steven G Nadler

Growth hormone & IGF research : official journal o 23:114-9 PubMed23651793

2013

Pegvisomant bioavailability of single 30 mg/mL subcutaneous injection compared to two 15 mg/mL subcutaneous injections: a pharmacokinetic, safety and tolerability study.

Applications

ELISA

Species

Unspecified reactive species

Juif Jen,Robert R LaBadie,Yali Liang,Penelope H Crownover,Xiang Gao,Juliana H Hey-Hadavi

Inflammatory bowel diseases 19:1224-31 PubMed23619715

2013

The cytotoxic effects of certolizumab pegol and golimumab mediated by transmembrane tumor necrosis factor α.

Applications

Unspecified application

Species

Unspecified reactive species

Naoyasu Ueda,Hiroshi Tsukamoto,Hiroki Mitoma,Masahiro Ayano,Atsushi Tanaka,Shun-ichiro Ohta,Yasushi Inoue,Yojiro Arinobu,Hiroaki Niiro,Koichi Akashi,Takahiko Horiuchi

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-Polyethylene glycol 抗体 [PEG-B-47b]

Biotin Anti-Polyethylene glycol antibody [PEG-B-47b]

ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)

ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)

Sandwich ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)

ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)

Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)

Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (AB53449)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

アプリケーション

免疫原

特異性

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (15)

Abcam product promise