AB79962

Biotin Anti-Neutrophil Elastase 抗体

Name: Biotin Anti-Neutrophil Elastase 抗体 (ab79962) | Abcam
Brand: Abcam Limited
SKU: AB79962
Price: 75000 JPY
Availability: InStock

Biotin Anti-Neutrophil Elastase antibody

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(2 Publications)

Sheep Polyclonal Neutrophil Elastase antibody - conjugated to Biotin. Suitable for ELISA and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human ELANE.

別名を表示する

ELA2, ELANE, Neutrophil elastase, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, Medullasin, PMN elastase, HLE

1 Images

ELISA - Biotin Anti-Neutrophil Elastase antibody (AB79962)

ELISA

Supplier Data

ELISA - Biotin Anti-Neutrophil Elastase antibody (AB79962)

Assay was performed using increasing concentrations of biotinylated purified human ELA2 protein. The antibody sensitivity is 30 pg/ml.

Key facts

宿主種

Sheep

クローン性

Polyclonal

アイソタイプ

IgG

標識

Biotin

励起波長/蛍光波長

キャリアフリー

交差種

Human

アプリケーション

ELISA

applications

免疫原

Native Full Length Protein corresponding to Human ELANE.

P08246

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.25% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Neutrophil Elastase also known as neutrophil proteinase 3 has a molecular mass of approximately 30 kDa. It is a serine protease expressed mainly in azurophilic granules of neutrophils. This enzyme breaks down various proteins including elastin at sites of inflammation. Neutrophil Elastase releases during degranulation or through neutrophil extracellular traps (NETs) to combat pathogens.

Biological function summary

The target plays a significant role in neutrophil-mediated tissue remodeling and host defense. It leads to the breaking down of extracellular matrix components contributing to tissue remodeling and repair. Neutrophil Elastase is part of a complex interplay with other proteases like matrix metalloproteinases (MMPs). Its activity is regulated by endogenous inhibitors such as alpha-1 antitrypsin to prevent excessive tissue damage.

Pathways

This protease is important in inflammation and immune response pathways. It plays a role in the complement system by activating C5 a component of the complement cascade. It also acts in conjunction with other proteases such as cathepsin G to modulate immune and inflammatory responses. Neutrophil Elastase's role within these pathways is pivotal for the regulation of inflammation and destruction of pathogens.

Individuals with conditions such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis often exhibit dysregulation or excessive activity of Neutrophil Elastase. This excessive activity leads to tissue damage and exacerbates disease progression. The enzyme also interacts with proteins like elastin and collagen in these diseases further contributing to pathological tissue remodeling and damage.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Serine protease that modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed : 15140022). Promotes cleavage of GSDMB, thereby inhibiting pyroptosis (PubMed : 36899106). Promotes blood coagulation (PubMed : 20676107). Through the activation of the platelet fibrinogen receptor integrin alpha-IIb/beta-3, potentiates platelet aggregation induced by a threshold concentration of cathepsin G (CTSG) (PubMed : 25211214, PubMed : 9111081). Cleaves and thus inactivates tissue factor pathway inhibitor (TFPI) (PubMed : 20676107, PubMed : 25211214). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed : 10947984).

See full target information ELANE

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38928283

2024

The Effect of Targeted Hyperoxemia on Brain Immunohistochemistry after Long-Term, Resuscitated Porcine Acute Subdural Hematoma and Hemorrhagic Shock.

Applications

Unspecified application

Species

Unspecified reactive species

Franziska Münz,Thomas Datzmann,Andrea Hoffmann,Michael Gröger,René Mathieu,Simon Mayer,Fabian Zink,Holger Gässler,Eva-Maria Wolfschmitt,Melanie Hogg,Enrico Calzia,Pierre Asfar,Peter Radermacher,Thomas Kapapa,Tamara Merz

JACC. Basic to translational science 3:435-449 PubMed30175268

2018

Ticagrelor Reduces Thromboinflammatory Markers in Patients With Pneumonia.

Applications

Unspecified application

Species

Unspecified reactive species

Travis R Sexton,Guoying Zhang,Tracy E Macaulay,Leigh A Callahan,Richard Charnigo,Olga A Vsevolozhskaya,Zhenyu Li,Susan Smyth

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-Neutrophil Elastase 抗体

Biotin Anti-Neutrophil Elastase antibody

ELISA - Biotin Anti-Neutrophil Elastase antibody (AB79962)

Key facts

宿主種

クローン性

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

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Abcam product promise