Biotin Anti-M13 抗体 [E1] (ab17269)
![ELISA - Biotin Anti-M13 antibody [E1] (ab17269)](https://www.abcam.co.jp/ps/products/17/ab17269/Images/ab17269-459706-biotin-anti-m13-antibody-e1-elisa.jpg "ELISA - Biotin Anti-M13 antibody [E1] (ab17269)")
Key features and details
- Biotin Mouse monoclonal [E1] to M13
- Suitable for: WB, ELISA, Flow Cyt, IHC
- Conjugation: Biotin
- Isotype: IgG3
製品の概要
-
製品名
Biotin Anti-M13 antibody [E1]
M13 一次抗体 製品一覧 -
製品の詳細
Biotin Mouse monoclonal [E1] to M13 -
由来種
Mouse -
標識
Biotin -
特異性
M13 antibody [E1] (ab17269) is specific for gp3 protein. -
アプリケーション
適用あり: WB, ELISA, Flow Cyt, IHCmore details -
種交差性
交差種: Enterobacteria phage M13 -
免疫原
Full length M13 phage coat protein; Exact epitope not determined.
-
特記事項
M13 antibody [E1] (ab17269) has numerous uses in screening phage display libraries, especially the screening of single chain antibody libraries.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
M13 antibody [E1] (ab17269) is purified using 0.2µm sterile filter. -
一次抗体 備考
M13 antibody [E1] (ab17269) has numerous uses in screening phage display libraries, especially the screening of single chain antibody libraries. -
ポリ/モノ
モノクローナル -
クローン名
E1 -
ミエローマ
unknown -
アイソタイプ
IgG3 -
軽鎖の種類
unknown -
研究分野
関連製品
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab17269の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 5 µg/ml.
|
|
| ELISA |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use 1µg for 106 cells.
ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
| IHC |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 - 5 µg/ml. |
|
ELISA
Use at an assay dependent concentration. |
|
Flow Cyt
Use 1µg for 106 cells. ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
IHC
Use at an assay dependent concentration. |
ターゲット情報
-
関連性
The bacteriophage known as "M13" forms the basis of cloning systems designed to easily introduce mutations into genes inserted into the phage genome. It also has been used in various "phage display" methodologies and combinatorial DNA and peptide libraries. Coat protein A (G3P) is necessary for adsorption of the virion onto the F-pilus of the host cell. There are about five copies of this protein per mature phage. Consists of three domains (N1, N2, and CT). The N2 domain interacts with the F pilus, whereas the N1 domain (connected to N2 by a flexible glycine-rich linker and tightly interacting with it on the phage) forms a complex with the C-terminal domain of tolA at later stages of the infection process. They are located at the adsorption end of the phage particle. -
別名
- Bacteriophage M13 antibody
- Coliphage M13 antibody
- Enterobacteria phage M13 antibody
see all
画像
-
ELISA - Biotin Anti-M13 antibody [E1] (ab17269)
ELISA absorbancy readings for A10B phage on rabbit IgG (A10B/IgG), A10B phage on BSA (A10B/BSA), streptavidin on rabbit IgG (SA/IgG) and streptavidin on BSA (SA/BSA) for each dilution of ab17269.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab17269 は論文での使用が確認できていません。