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AB20766

Biotin Anti-Listeria monocytogenes 抗体

Biotin Anti-Listeria monocytogenes antibody

4

(2 Reviews)

|

(1 Publication)

Anti-Listeria monocytogenes antibody conjugated to Biotin is a rabbit polyclonal antibody suitable for ELISA, IHC-Fr.

別名を表示する

L. monocytogenes

1 Images
Immunohistochemistry (Frozen sections) - Biotin Anti-Listeria monocytogenes antibody (AB20766)
  • IHC-Fr

AbReview14348****

Immunohistochemistry (Frozen sections) - Biotin Anti-Listeria monocytogenes antibody (AB20766)

ab20766 staining Listeria monocytogenes in mouse spleen tissue section from a mice infected with 1x10exp6 L. monocytogenes for 24h by Immunohistochemistry (Frozen sections). Tissue samples were fixed with 4% paraformaldehyde for 2 hours at RT and then it was incubated ON in 30% Sucrose in PBS at 4°C, froze away in OCT/Tissuetek. The tissue samples were blocked with 1% 1 : 20 Cd16/32 at 200C for 30 minutes. The sample was incubated with primary antibody (1/250) in PBS at 200C for 1 hour. For detection Streptavidin-Cy3 was used at dilution at 1 : 500 in PBS for 30 min at 20°C.

This image is a courtesy of Anonymous Abreview

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

標識

Biotin

励起波長/蛍光波長
キャリアフリー

No

交差種

Listeria monocytogenes

アプリケーション

IHC-Fr, ELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Listeria monocytogenes": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/1000", "ELISA-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/250", "IHCFr-species-notes": "<p></p>" } } }

製品の詳細

IgG fraction covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.

出荷温度及び保存条件

製品の状態
Liquid
精製度
IgG fraction
バッファー組成
Preservative: 0.1% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Avoid freeze / thaw cycle

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 5:e15567 PubMed21179567

2010

A fluorescence reporter model defines "Tip-DCs" as the cellular source of interferon β in murine listeriosis.

Applications

IHC-Fr

Species

Unspecified reactive species

Philipp Dresing,Stephanie Borkens,Magdalena Kocur,Sonja Kropp,Stefanie Scheu
View all publications

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