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Biotin Anti-IRE1 (phospho S724) 抗体 (ab81936)

Reviews (1)Q&A (1)References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-IRE1 (phospho S724) antibody (ab81936)

    Key features and details

    • Biotin Rabbit polyclonal to IRE1 (phospho S724)
    • Suitable for: IHC-P
    • Reacts with: Mouse, Rat, Human
    • Conjugation: Biotin
    • Isotype: IgG

    製品の概要

    • 製品名

      Biotin Anti-IRE1 (phospho S724) antibody
      IRE1 一次抗体 製品一覧

    • 製品の詳細

      Biotin Rabbit polyclonal to IRE1 (phospho S724)

    • 由来種

      Rabbit

    • 標識

      Biotin

    • アプリケーション

      適用あり: IHC-Pmore details

    • 種交差性

      交差種: Mouse, Rat, Human
      非交差種: Monkey

    • 免疫原

      Synthetic peptide corresponding to Human IRE1 (phospho S724).

    • ポジティブ･コントロール

      • Glucose treated pancreatic islets and endogenous protein of pancreatic beta cells and insuli. COS-7 expressing wild-type IRE1. Min6 cells treated with glucose.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      Constituents: 30% Glycerol, PBS
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab81936の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P
    1/300.
    特記事項
    IHC-P
    1/300.

    ターゲット情報

    • 機能

      Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

    • 組織特異性

      Ubiquitously expressed. High levels observed in pancreatic tissue.

    • 配列類似性

      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
      Contains 1 KEN domain.
      Contains 1 protein kinase domain.

    • 翻訳後修飾

      Autophosphorylated.

    • 細胞内局在

      Endoplasmic reticulum membrane.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
      • Endoplasmic reticulum to nucleus signaling 1 antibody
      • Endoplasmic reticulum-to-nucleus signaling 1 antibody
      • Endoribonuclease antibody
      • ER to nucleus signaling 1 antibody
      • ERN 1 antibody
      • Ern1 antibody
      • ERN1_HUMAN antibody
      • hIRE 1p antibody
      • hIRE1p antibody
      • Inositol requiring 1 antibody
      • Inositol requiring 1, S. cerevisiae, homolog of antibody
      • Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody
      • Inositol requiring protein 1 antibody
      • inositol-requiring enzyme 1 antibody
      • Inositol-requiring protein 1 antibody
      • IRE 1 antibody
      • IRE 1a antibody
      • IRE 1P antibody
      • Ire1 alpha antibody
      • Ire1-alpha antibody
      • IRE1a antibody
      • Ire1alpha antibody
      • IRE1P antibody
      • MGC163277 antibody
      • MGC163279 antibody
      • Protein kinase/endoribonuclease antibody
      • RGD1559716 antibody
      • Serine/threonine protein kinase/endoribonuclease IRE1 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-IRE1 (phospho S724) antibody (ab81936)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-IRE1 (phospho S724) antibody (ab81936)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling IRE1 with the unconjugated version of at81936 at 1/300 dilution. 

    参考文献 (1)

    ab81936 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab81936 は 1 報の論文で使用されています。

    • He Y  et al. Palmitic acid, but not high-glucose, induced myocardial apoptosis is alleviated by N-acetylcysteine due to attenuated mitochondrial-derived ROS accumulation-induced endoplasmic reticulum stress. Cell Death Dis 9:568 (2018). PubMed: 29752433

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-IRE1 (phospho S724) antibody (Biotin)

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (AR42J)
    Permeabilization
    Yes - 10% Triton x100
    Specification
    AR42J
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jul 24 2017

    Question

    Would like to try this antibody with rat samples.

    Read More
    Answer

    Thank you very much for your call today.
    DISCOUNT CODE: ***
    Expiration date: December 14, 2012
    This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab104157 with rat samples and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
    The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

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