AB95584

Biotin Anti-Integrin beta 4 抗体 [439-9B]

Name: Biotin Anti-Integrin beta 4 抗体 [439-9B] (ab95584) | Abcam
Brand: Abcam Limited
SKU: AB95584
Price: 38000 JPY
Availability: InStock

Biotin Anti-Integrin beta 4 antibody [439-9B]

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(2 Publications)

Anti-Integrin beta 4 antibody [439-9B] conjugated to Biotin - suitable for flow cytometry, and reacts with human samples. Clone [439-9B] is a rat monoclonal antibody cited in 7 publications.

別名を表示する

CD104, Integrin beta-4, GP150, ITGB4

1 Images

Flow Cytometry - Biotin Anti-Integrin beta 4 antibody [439-9B] (AB95584)

Flow Cyt

Unknown

Flow Cytometry - Biotin Anti-Integrin beta 4 antibody [439-9B] (AB95584)

Staining of the A549 cell line with 0.125 µg of Rat IgG2b kappa Isotype Control Biotin (open histogram) or 0.125 µg of ab95584 (filled histogram) followed by Streptavidin PE. Total viable cells were used for analysis.

Key facts

宿主種

Rat

クローン性

Monoclonal

クローン番号

439-9B

アイソタイプ

IgG2b

標識

Biotin

励起波長/蛍光波長

キャリアフリー

交差種

Human

アプリケーション

Flow Cyt

applications

免疫原

Full Length Protein corresponding to Human ITGB4. The exact immunogen used to generate this antibody is proprietary information.

P16144

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.25 µg for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/biotin-rat-igg2b-kappa-monoclonal-rtk4530-isotype-control-ab18542'>ab18542</a> - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }

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製品の詳細

ab95584 has been reported to have no effect on adhesion of Human colon adenocarcinoma cells

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

バッファー組成

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Integrin beta 4 also known as ITGB4 integrin ß4 or integrine beta 4 is a transmembrane protein that mechanically links the extracellular matrix to the intracellular cytoskeleton. This protein has a molecular mass of approximately 205 kDa. Integrin beta 4 primarily expresses in epithelial cells particularly those forming stratified and transitional epithelia. It partners with alpha 6 integrin to form the alpha 6 beta 4 integrin complex which plays a significant role in the formation and maintenance of hemidesmosomes.

Biological function summary

Integrin beta 4 is important for anchoring epithelial cells to the basement membrane stabilizing cell layers in tissues. It interacts with intermediate filaments through specific cytoplasmic proteins. The integrin beta 4 complex ensures structural integrity essential for maintaining the skin and mucosal barriers. Integrations with proteins like M126 are essential for strengthening these interactions safeguarding cellular stability and promoting wound healing.

Pathways

Integrin beta 4 is embedded in key signaling pathways that influence cell adhesion and migration. It participates prominently in the PI3K/AKT and RAS pathway collaborations facilitating signal transduction essential for cell growth and survival. Through these pathways integrin beta 4 interacts with proteins like IS200BPIRG1A linking extracellular cues to internal cellular responses.

Integrin beta 4 has associations with epidermolysis bullosa and various cancers. In epidermolysis bullosa mutations in integrin beta 4 impair hemidesmosomal function leading to skin fragility. In cancer integrin beta 4 overexpression or mislocalization contributes to tumor progression affecting cell invasion and metastasis. Its interactions with IS200BPIAG1A or related pathways highlight the protein’s influence on carcinogenic processes and potential as a therapeutic target.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6 : ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGA6 : ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 22351760). ITGA6 : ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464).

See full target information ITGB4

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 107 ( Pt 3):577-87 PubMed8006072

1994

Adhesive properties and integrin expression profiles of two colonic cancer populations differing by their spreading on laminin.

Applications

Unspecified application

Species

Unspecified reactive species

P Simon-Assmann,C Leberquier,N Molto,T Uezato,F Bouziges,M Kedinger

Cancer research 48:816-21 PubMed2448027

1988

Monoclonal antibody to human carcinoma-associated protein complex: quantitation in normal and tumor tissue.

Applications

Unspecified application

Species

Unspecified reactive species

R Falcioni,A Sacchi,J Resau,S J Kennel

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-Integrin beta 4 抗体 [439-9B]

Biotin Anti-Integrin beta 4 antibody [439-9B]

Flow Cytometry - Biotin Anti-Integrin beta 4 antibody [439-9B] (AB95584)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

Abcam product promise