Biotin Anti-IL-4 抗体 (ab84278)
Key features and details
- Biotin Rabbit polyclonal to IL-4
- Suitable for: Sandwich ELISA, WB
- Reacts with: Rat
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-IL-4 antibody
IL-4 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to IL-4 -
由来種
Rabbit -
標識
Biotin -
アプリケーション
適用あり: Sandwich ELISA, WBmore details -
種交差性
交差種: Rat -
免疫原
Recombinant full length protein corresponding to Rat IL-4.
Database link: P20096 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: PBS -
Concentration information loading... -
特記事項（精製）
ab84278 was purified by affinity chromatography and then biotinylated.It is sterile filtered. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84278の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use a concentration of 0.25 - 1 µg/ml.
Use 100 μl/well antibody solution. Detects at least 0.2 – 0.4 ng/well of recombinant rat IL-4. |
|
| WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 16 kDa.
Detection limit for recombinant rat IL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| 特記事項 |
|---|
|
Sandwich ELISA
Use a concentration of 0.25 - 1 µg/ml. Use 100 μl/well antibody solution. Detects at least 0.2 – 0.4 ng/well of recombinant rat IL-4. |
|
WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 16 kDa. Detection limit for recombinant rat IL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
ターゲット情報
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機能
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. -
関連疾患
Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. -
配列類似性
Belongs to the IL-4/IL-13 family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 287287 Rat
- SwissProt: P20096 Rat
- Unigene: 108255 Rat
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別名
- B cell growth factor 1 antibody
- B cell IgG differentiation factor antibody
- B Cell Stimulatory Factor 1 antibody
see all
画像
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Western blot - Biotin Anti-IL-4 antibody (ab84278)
To detect rat IL-4 by Western Blot analysis ab84278 can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant rat IL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is non-reducing conditions; Rat recombinant IL-4 protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 rat recombinant IL-4 protein.
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Sandwich ELISA - Biotin Anti-IL-4 antibody (ab84278)To detect rat IL-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab84278 is required. Using this antibody in conjunction with an anti-IL-4 (ab9811) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant rat IL-4.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab84278 は論文での使用が確認できていません。
