Biotin Anti-IGFBP1 抗体 (ab246315)
Key features and details
- Biotin Rabbit polyclonal to IGFBP1
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-IGFBP1 antibody
IGFBP1 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to IGFBP1 -
由来種
Rabbit -
標識
Biotin -
アプリケーション
適用あり: WB, Sandwich ELISAmore details -
種交差性
交差種: Recombinant fragment
交差が予測される動物種: Human
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免疫原
Recombinant full length protein corresponding to Human IGFBP1 aa 1 to the C-terminus. Produce in E. coli. Full length mature chain without signal peptide.
Database link: P08833 -
ポジティブ･コントロール
- WB: Recombinant human IGFBP1 protein. sELISA: Recombinant human IGFBP1 protein.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab246315の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.2 µg/ml.
|
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| Sandwich ELISA |
Use a concentration of 0.25 - 1 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.2 µg/ml. |
|
Sandwich ELISA
Use a concentration of 0.25 - 1 µg/ml. |
ターゲット情報
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機能
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. -
配列類似性
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
翻訳後修飾
Phosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3484 Human
- Omim: 146730 Human
- SwissProt: P08833 Human
- Unigene: 642938 Human
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別名
- AFBP antibody
- Alpha pregnancy associated endometrial globulin antibody
- Amniotic fluid binding protein antibody
see all
画像
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Western blot - Biotin Anti-IGFBP1 antibody (ab246315)
To detect human IGFBP1 by Western Blot analysis ab246315 can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human IGFBP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane 1: Marker.
Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng/lane respectively.
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Sandwich ELISA - Biotin Anti-IGFBP1 antibody (ab246315)To detect human IGFBP1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab246315 is required. This biotinylated polyclonal antibody, in conjunction with a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human IGFBP1.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab246315 は論文での使用が確認できていません。
