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AB201248

Biotin Anti-Human IgG3 抗体 [EPR4419]

Biotin Anti-Human IgG3 antibody [EPR4419]

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(1 Publication)

Rabbit Recombinant Monoclonal IGHG3 antibody - conjugated to Biotin. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

別名を表示する

Immunoglobulin heavy constant gamma 3, HDC, Heavy chain disease protein, Ig gamma-3 chain C region, IGHG3

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Human IgG3 antibody [EPR4419] (AB201248)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Human IgG3 antibody [EPR4419] (AB201248)

Negative IHC image of human IgG3 staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex*, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab201248, at 1/50 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

The inset background control image is taken from an identical assay without added antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Human IgG3 antibody [EPR4419] (AB201248)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Human IgG3 antibody [EPR4419] (AB201248)

IHC image of human IgG3 staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab201248, at 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

The inset background control image is taken from an identical assay without added antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4419

アイソタイプ

IgG

標識

Biotin

励起波長/蛍光波長
キャリアフリー

No

交差種

Human, Human

アプリケーション

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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代替製品

Primary Antibodies

AB1928

Anti-Human IgG3 antibody [5G12]

  • 0
  • 0
Primary Antibodies

AB193172

Anti-Human IgG3 antibody [RM119]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG3 antibody [RM119] (AB193172)

  • 0
  • 0

組合せ製品

Assay Kits

AB318929

Human IgG3 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

  • 0
  • 0
Secondary Antibodies

AB1927

Mouse monoclonal [2C11] Anti-Human IgG1 Fc

ELISA - Mouse monoclonal [2C11] Anti-Human IgG1 Fc (AB1927)

  • 5
  • 1
Secondary Antibodies

AB99817

Mouse Anti-Human IgG4 pFc’ [HP6023] (HRP)

ELISA - Mouse Anti-Human IgG4 pFc’ [HP6023] (HRP) (AB99817)

  • 0
  • 0
Primary Antibodies

AB193172

Anti-Human IgG3 antibody [RM119]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG3 antibody [RM119] (AB193172)

  • 0
  • 0
Primary Antibodies

AB201485

Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Biotin)

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Biotin) (AB201485)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Stable for 12 months at -20°C|Store in the dark
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).
See full target information IGHG3
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 9:2603-2611 PubMed30087700

2018

microRNA-485-5p Functions as a Tumor Suppressor in Colorectal Cancer Cells by Targeting CD147.

Applications

Unspecified application

Species

Unspecified reactive species

Xiu-Xiu Hu,Xue-Ni Xu,Bang-Shun He,Hui-Ling Sun,Tao Xu,Xiang-Xiang Liu,Xiao-Xiang Chen,Kai-Xuan Zeng,Shu-Kui Wang,Yu-Qin Pan
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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