JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB314264

Biotin Anti-GFAP 抗体 [EPR1034Y]

Biotin Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal GFAP antibody - conjugated to Biotin. Suitable for IHC-P, WB and reacts with Mouse, Human, Rat samples.

別名を表示する

Glial fibrillary acidic protein, GFAP

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB314264)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB314264)

Negative staining of GFAP in a section of formalin-fixed paraffin-embedded mouse liver performed on a Leica BOND™ system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab314264 at 5 ug/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB314264)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB314264)

IHC image of GFAP staining in a section of formalin-fixed paraffin-embedded mouse brain performed on a Leica BOND™ system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab314264 at 5 ug/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

関連する標識済み抗体及び組成の異なる製品 (11)

  • 421 Alexa Fluor® 405

    Alexa Fluor® 405 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

  • 578 PE

    PE Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

  • 660 APC

    APC Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

  • HRP

    HRP Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

  • Unconjugated

    Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

  • Carrier free

    Anti-GFAP antibody [EPR1034Y] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR1034Y

アイソタイプ

IgG

標識

Biotin

励起波長/蛍光波長
キャリアフリー

No

交差種

Mouse, Human, Mouse, Rat

アプリケーション

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
style
left-column
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
See full target information GFAP
style
left-column
style
left-column
style
right-column

組合せ製品

Assay Kits

AB233621

Mouse GFAP ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse GFAP ELISA Kit (AB233621)

  • 0
  • 0
Primary Antibodies

AB190565

Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB190565)

  • 5
  • 7
Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

  • 5
  • 50
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB52642

Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB52642)

  • 4
  • 37

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com