Biotin Anti-Cytokeratin 18 抗体 [DC-10] (ab27551)
Key features and details
- Biotin Mouse monoclonal [DC-10] to Cytokeratin 18
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
製品の概要
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製品名
Biotin Anti-Cytokeratin 18 antibody [DC-10]
Cytokeratin 18 一次抗体 製品一覧 -
製品の詳細
Biotin Mouse monoclonal [DC-10] to Cytokeratin 18 -
由来種
Mouse -
標識
Biotin -
特異性
ab27551 recognises cytokeratin peptide 18 (45 kDa) in human tissue. -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human Cytokeratin 18. Human breast carcinoma cell line PMC-42
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ポジティブ・コントロール
- Flow Cyt (Intra): HeLa human cervix carcinoma cell. WB: HT-29 (reducing and non-reducing conditions) cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
IgG fraction -
ポリ/モノ
モノクローナル -
クローン名
DC-10 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab27551の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
Use a concentration of 2 - 6 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 48 kDa.
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特記事項 |
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Flow Cyt (Intra)
Use a concentration of 2 - 6 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 48 kDa. |
ターゲット情報
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機能
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. -
組織特異性
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. -
関連疾患
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. -
細胞内局在
Cytoplasm > perinuclear region. - Information by UniProt
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参照データベース
- Entrez Gene: 3875 Human
- Omim: 148070 Human
- SwissProt: P05783 Human
- Unigene: 406013 Human
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別名
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
- Cell proliferation-inducing gene 46 protein antibody
see all
画像
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Flow Cytometry analysis (intracellular staining) of HeLa cells labeling Cytokeratin 18 with ab27551 and streptavidin APC at 6 µg / ml concentration (red), and blank sample (black)
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All lanes : Biotin Anti-Cytokeratin 18 antibody [DC-10] (ab27551) at 2 µg/ml
Lane 1 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (reducing conditions)
Lanes 2-3 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (reducing conditions)
Lane 4 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (non-reducing conditions)
Predicted band size: 48 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab27551 は論文での使用が確認できていません。