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AB27553

Biotin Anti-Cytokeratin 18 抗体 [C-04]

Biotin Anti-Cytokeratin 18 antibody [C-04]

5

(2 Reviews)

|

(6 Publications)

Anti-Cytokeratin 18 antibody [C-04] conjugated to Biotin - suitable for Western blot, and reacts with human samples. Clone [C-04] is a mouse monoclonal antibody cited in 10 publications.

別名を表示する

CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

C-04

アイソタイプ

IgG1

標識

Biotin

励起波長/蛍光波長
キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

Cell preparation containing KRT18 protein. The exact immunogen used to generate this antibody is proprietary information.

P05783

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代替製品

Primary Antibodies

AB206091

Alexa Fluor® 488 Anti-Cytokeratin 18 antibody [EPR1626]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 18 antibody [EPR1626] (AB206091)

  • 0
  • 0
Primary Antibodies

AB133302

Anti-Cytokeratin 18 antibody [EPR1611]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [EPR1611] (AB133302)

  • 0
  • 0
Primary Antibodies

AB181597

Anti-Cytokeratin 18 antibody [EPR17347]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

  • 5
  • 3
Primary Antibodies

AB133263

Anti-Cytokeratin 18 antibody [EPR1626]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR1626] (AB133263)

  • 3
  • 3
Primary Antibodies

AB32118

Anti-Cytokeratin 18 antibody [E431-1]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [E431-1] (AB32118)

  • 3
  • 6
Primary Antibodies

AB194124

Alexa Fluor® 488 Anti-Cytokeratin 18 antibody [E431-1]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 18 antibody [E431-1] (AB194124)

  • 0
  • 0

組合せ製品

Assay Kits

AB254515

Human Cytokeratin 18 Fragment ELISA Kit (CK18-30)

Recombinant|SimpleStep

ELISA - Human Cytokeratin 18 Fragment ELISA Kit (CK18-30) (AB254515)

  • 0
  • 0
Primary Antibodies

AB192467

Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y] (AB192467)

  • 5
  • 5
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
Primary Antibodies

AB237385

Alexa Fluor® 647 Anti-EpCAM antibody [EPR20533-63]

RabMAb|Recombinant

Flow Cytometry - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20533-63] (AB237385)

  • 0
  • 0
Primary Antibodies

AB246727

Alexa Fluor® 488 Anti-p63 antibody [EPR5701]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-p63 antibody [EPR5701] (AB246727)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Mammals": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Size-exclusion chromatography
精製に関する特記事項
Purity >95% by SDS-PAGE.
バッファー組成
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.
Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Required for the formation of KRT8/KRT18 filaments that are involved in ARHGEF40-mediated actin stress fiber formation and tensional force-induced stress fiber formation and reinforcement (PubMed : 26823019). Also acts downstream of ROCK kinase activation as part of a positive feedback mechanism in response to cellular mechanical stress loading (PubMed : 26823019). Organization and orientation of KRT18 filaments are responsible for the properly elongated morphology of epithelial tubules (By similarity). Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
See full target information KRT18
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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:2177-2191 PubMed40858905

2025

ERG-driven prostate cancer initiation is cell-context dependent and requires KMT2A and DOT1L.

Applications

Unspecified application

Species

Unspecified reactive species

Weiran Feng,Erik Ladewig,Matthew Lange,Nazifa Salsabeel,Huiyong Zhao,Young Sun Lee,Anuradha Gopalan,Hanzhi Luo,Wenfei Kang,Ning Fan,Eric Rosiek,Elisa de Stanchina,Yu Chen,Brett S Carver,Christina S Leslie,Charles L Sawyers

Cell reports 41:111641 PubMed36384107

2022

A dietary change to a high-fat diet initiates a rapid adaptation of the intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Jacob R Enriquez,Heather A McCauley,Kevin X Zhang,J Guillermo Sanchez,Gregory T Kalin,Richard A Lang,James M Wells

Oncotarget 8:93899-93911 PubMed29212197

2017

Paeoniflorin prevents postoperative peritoneal adhesion formation in an experimental rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Gao,Guangbing Wei,Yunhua Wu,Na Yao,Cancan Zhou,Kai Wang,Kang Wang,Xuejun Sun,Xuqi Li

Nature cell biology 16:942-50, 1-7 PubMed25173976

2014

Mammary stem cells have myoepithelial cell properties.

Applications

IHC-P

Species

Mouse

Michael D Prater,Valérie Petit,I Alasdair Russell,Rajshekhar R Giraddi,Mona Shehata,Suraj Menon,Reiner Schulte,Ivo Kalajzic,Nicola Rath,Michael F Olson,Daniel Metzger,Marisa M Faraldo,Marie-Ange Deugnier,Marina A Glukhova,John Stingl

PloS one 8:e51671 PubMed23300950

2013

FGFR2 promotes breast tumorigenicity through maintenance of breast tumor-initiating cells.

Applications

IHC-P

Species

Mouse

Sungeun Kim,Anna Dubrovska,Richard J Salamone,John R Walker,Kathryn B Grandinetti,Ghislain M C Bonamy,Anthony P Orth,Jimmy Elliott,Diana Graus Porta,Carlos Garcia-Echeverria,Venkateshwar A Reddy

Stem cells (Dayton, Ohio) 26:2245-56 PubMed18556507

2008

Scalable selection of hepatocyte- and hepatocyte precursor-like cells from culture of differentiating transgenically modified murine embryonic stem cells.

Applications

ICC/IF

Species

Unspecified reactive species

Irina Drobinskaya,Thomas Linn,Tomo Saric,Reinhard G Bretzel,Heribert Bohlen,Jürgen Hescheler,Eugen Kolossov
View all publications
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