Biotin Anti-CD45 抗体 [MEM-28]

• Flow Cyt

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Flow Cytometry - Biotin Anti-CD45 antibody [MEM-28] (AB28107)

Flow Cytometry analysis of Human peripheral blood cells labeling CD45 with Anti-CD45 antibody [MEM-28] (Biotin) (ab28107).

• WB

Supplier Data

Western blot - Biotin Anti-CD45 antibody [MEM-28] (AB28107)

Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, Raji, HeLa, and HEK293T cell lines, mixed and heated (100°C, 5 min) with non-reducing SDS-loading buffer. Samples were resolved using 7% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with ab28107 (1 µg/ml), followed by IRDye 800CW Streptavidin (green). Multiplex fluorescent Western blot detection was performed. CD45 molecules were detected at ~180-250 kDa in Jurkat and Raji cell lines

All lanes:

Western blot - Biotin Anti-CD45 antibody [MEM-28] (ab28107)

Lanes 1 and 4:

Jurkat whole cell extract with non-reducing SDS-loading buffer

Lane 2:

Raji whole cell extract with non-reducing SDS-loading buffer

Lane 3:

HeLa whole cell extract with non-reducing SDS-loading buffer

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