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AB191112

Biotin Anti-CD3 抗体 [UCHT1]

Biotin Anti-CD3 antibody [UCHT1]

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(2 Publications)

Anti-CD3 antibody [UCHT1] conjugated to Biotin - suitable for flow cytometry, intracellular flow cytometry, and reacts with human samples. Clone [UCHT1] is a mouse monoclonal antibody and one of the most commonly used clones for flow cytometry.

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別名を表示する

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

2 Images
Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (AB191112)
  • Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (AB191112)

Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab191112 (concentration in sample 0.3 µg/mL) streptavidin APC.

Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (AB191112)
  • Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (AB191112)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab191112 (concentration in sample 0.3 µg/mL) streptavidin APC.

関連する標識済み抗体及び組成の異なる製品 (13)

  • 660 APC

    APC Anti-CD3 antibody [UCHT1]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD3 antibody [UCHT1]

  • 519 FITC

    FITC Anti-CD3 antibody [UCHT1]

  • 519 FITC

    FITC Anti-CD3 epsilon antibody [UCHT1]

  • 578 PE

    PE Anti-CD3 epsilon antibody [UCHT1]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD3 antibody [UCHT1]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD3 antibody [UCHT1]

  • 675 PerCP

    PerCP Anti-CD3 antibody [UCHT1]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD3 antibody [UCHT1]

  • 387 mFluor™ UV375

    mFluor™ UV375 Anti-CD3e antibody [UCHT1]

  • 454 mFluor™ Violet 450

    mFluor™ Violet 450 Anti-CD3e antibody [UCHT1]

  • 704 redFluor™ 710

    redFluor™ 710 Anti-CD3 antibody [UCHT1]

  • 450 violetFluor™ 450

    violetFluor™ 450 Anti-CD3 antibody [UCHT1]

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

UCHT1

アイソタイプ

IgG1

標識

Biotin

励起波長/蛍光波長
キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt

applications

免疫原

Cell preparation containing CD3D protein. The exact immunogen used to generate this antibody is proprietary information.

P04234

特異性

ab191112 recognizes the CD3 antigen of the TCR/CD3 complex on mature Human T cells. The antibody reacts with the epsilon chain of the CD3 complex.

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代替製品

Primary Antibodies

AB5690

Anti-CD3 epsilon antibody

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody (AB5690)

  • 4
  • 46
Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

  • 4
  • 80
Primary Antibodies

AB21703

Anti-CD3 epsilon antibody [SP7], prediluted

BOND RX™ Validated|RabMAb|Lab Essentials|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7], prediluted (AB21703)

  • 5
  • 1
Primary Antibodies

AB246799

Anti-CD3 antibody [SP162] - Low endotoxin, Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD3 antibody [SP162] - Low endotoxin, Azide free (AB246799)

  • 0
  • 0
Primary Antibodies

AB245731

Anti-CD3 antibody [SP162] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD3 antibody [SP162] - BSA and Azide free (AB245731)

  • 5
  • 3
Primary Antibodies

AB135372

Anti-CD3 antibody [SP162]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP162] (AB135372)

  • 4
  • 10
Primary Antibodies

AB16044

Anti-CD3 antibody

Immunoprecipitation - Anti-CD3 antibody (AB16044)

  • 4
  • 2

組合せ製品

Primary Antibodies

AB305048

Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66] (AB305048)

  • 5
  • 1
Primary Antibodies

AB200315

Alexa Fluor® 488 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD45 antibody [EP322Y] (AB200315)

  • 0
  • 0
Primary Antibodies

AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

  • 5
  • 1
Primary Antibodies

AB237456

Alexa Fluor® 647 Anti-NCAM1 antibody [EP2567Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NCAM1 antibody [EP2567Y] (AB237456)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-12 µg/mL", "FlowCyt-species-notes": "<p></p>" }, "Primates": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Size-exclusion chromatography
精製に関する特記事項
ab191112 is free of unconjugated biotin.
バッファー組成
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).
See full target information CD3D
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Biosensors 12: PubMed35049640

2021

Development of a Microfluidic Device for CD4 T Cell Isolation and Automated Enumeration from Whole Blood.

Applications

Unspecified application

Species

Unspecified reactive species

Robert D Fennell,Mazhar Sher,Waseem Asghar

The Journal of experimental medicine 178:1121-6 PubMed7688788

1993

A novel 80-kD cell surface structure identifies human circulating lymphocytes with natural killer activity.

Applications

Unspecified application

Species

Unspecified reactive species

H Maïza,G Leca,I G Mansur,V Schiavon,L Boumsell,A Bensussan
View all publications
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