AB21901

Biotin Anti-CD2 抗体 [MEM-65]

Biotin Anti-CD2 antibody [MEM-65]

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(1 Publication)

Anti-CD2 antibody [MEM-65] conjugated to Biotin - suitable for flow cytometry, and reacts with human samples. Clone [MEM-65] is a mouse monoclonal antibody.

別名を表示する

CD2, SRBC, T-cell surface antigen CD2, Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, T-cell surface antigen T11/Leu-5

1 Images

Flow Cytometry - Biotin Anti-CD2 antibody [MEM-65] (AB21901)

Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-CD2 antibody [MEM-65] (AB21901)

Surface staining of human peripheral blood with ab21901, streptavidin-APC.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

MEM-65

アイソタイプ

IgG1

標識

Biotin

励起波長/蛍光波長

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt

免疫原

Cell preparation containing CD2 protein. The exact immunogen used to generate this antibody is proprietary information.

特異性

This antibody recognises a unique epitope of CD2 antigen implicated in T cell activation.

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代替製品

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Primary Antibodies

AB269318

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組合せ製品

Primary Antibodies

AB305048

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Primary Antibodies

AB196147

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Primary Antibodies

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Primary Antibodies

AB208514

Alexa Fluor® 555 Anti-CD3D antibody [EP4426]

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4
2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-5 µg/mL", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/biotin-mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab18434'>ab18434</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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製品の詳細

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

バッファー組成

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD2 also known as T11 or LFA-2 acts as a cell surface glycoprotein expressed mainly on T-cells natural killer cells and thymocytes. This protein has a molecular mass of approximately 50-55 kDa. CD2 plays a critical role in facilitating cell-cell adhesion and signal transduction. It serves as a receptor for the CD58 ligand on antigen-presenting cells supporting the interaction between T-cells and these cells. By enhancing adhesion CD2 contributes to the formation of the immunological synapse necessary for effective immune response.

Biological function summary

The functioning of CD2 helps in the modulation of immune responses and T-cell activation. CD2 participates in signal transduction leading to cytokine production and proliferation. It is associated with the immunological synapse complex working closely with other surface molecules like Lck and CD3 to promote T-cell responses. This collaboration is vital for the development and function of immune cells driving adaptive immunity.

Pathways

CD2 engages in pathways integral to T-cell activation and signal transduction. A well-known pathway involving CD2 is the TCR (T-cell Receptor) signaling pathway where it partners with CD3 to initiate T-cell activation. Additionally CD2 influences the LAT (Linker for Activation of T-cells) pathway contributing to downstream signaling cascades that involve various signaling proteins such as Lck and ZAP-70 ensuring proper T-cell function.

CD2 shows connections to autoimmune diseases and cancers. For instance its involvement in abnormal T-cell activation links CD2 to autoimmune conditions like rheumatoid arthritis. Also various leukemias demonstrate altered CD2 expression suggesting a role in cancer pathogenesis. Through these disease states CD2's interaction with the CD58 protein becomes critical as this interaction affects the activity of T-cells in the disease milieu influencing disease progression and treatment outcomes.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

See full target information CD2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed36125261

2022

Progressive enhancement of kinetic proofreading in T cell antigen discrimination from receptor activation to DAG generation.

Applications

Unspecified application

Species

Unspecified reactive species

Derek M Britain,Jason P Town,Orion David Weiner

View all publications

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