AB28118

Biotin Anti-CD14 抗体 [MEM-15]

Name: Biotin Anti-CD14 抗体 [MEM-15] (ab28118) | Abcam
Brand: Abcam Limited
SKU: AB28118
Price: 75000 JPY
Availability: InStock

Biotin Anti-CD14 antibody [MEM-15]

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(1 Publication)

Mouse Monoclonal CD14 antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Human CD14.

別名を表示する

CD14, Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein

1 Images

Flow Cytometry - Biotin Anti-CD14 antibody [MEM-15] (AB28118)

Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-CD14 antibody [MEM-15] (AB28118)

Analysis by flow cytometry of ab28118 staining CD14 in human peripheral blood cells.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

MEM-15

アイソタイプ

IgG1

軽鎖のタイプ

kappa

標識

Biotin

励起波長/蛍光波長

キャリアフリー

交差種

Human

アプリケーション

Flow Cyt

applications

免疫原

Native Full Length Protein corresponding to Human CD14.

P08571

特異性

ab28118 recognises CD14 expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. ab28118 also reacts with soluble forms of CD14 found in serum and in the urine of some nephrotic patients.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2-5 µg/mL", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/biotin-mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab18434'>ab18434</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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製品の詳細

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

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出荷温度及び保存条件

製品の状態

Liquid

精製に関する特記事項

The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

バッファー組成

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.

Biological function summary

This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.

Pathways

CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.

Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

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ターゲットの情報

Coreceptor for bacterial lipopolysaccharide (PubMed : 1698311, PubMed : 23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed : 20133493, PubMed : 22265692, PubMed : 23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 8612135). Acts as a coreceptor for TLR2 : TLR6 heterodimer in response to diacylated lipopeptides and for TLR2 : TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed : 16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed : 23880187).

See full target information CD14

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Folia biologica 32:12-25 PubMed2422063

1986

Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen.

Applications

Unspecified application

Species

Unspecified reactive species

V Horejsí,I Hilgert,H Kristofová,V Bazil,A Bukovský,J Kulhánková

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-CD14 抗体 [MEM-15]

Biotin Anti-CD14 antibody [MEM-15]

Flow Cytometry - Biotin Anti-CD14 antibody [MEM-15] (AB28118)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

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Abcam product promise