AB6645

Biotin Anti-beta Galactosidase 抗体

Name: Biotin Anti-beta Galactosidase 抗体 (ab6645) | Abcam
Brand: Abcam Limited
SKU: AB6645
Price: 74000 JPY
Availability: InStock

Biotin Anti-beta Galactosidase antibody

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(4 Publications)

Rabbit Polyclonal GLB1/Beta-galactosidase antibody - conjugated to Biotin. Suitable for ELISA, IHC-P, WB, Dot and reacts with Escherichia coli, Recombinant full length protein - Escherichia coli samples. Cited in 4 publications.

別名を表示する

b0344, JW0335, lacZ, Beta-galactosidase, Beta-gal, Lactase

1 Images

Supplier Data

Western blot - Biotin Anti-beta Galactosidase antibody (AB6645)

All lanes:

Western blot - Biotin Anti-beta Galactosidase antibody (ab6645) at 1/1000 dilution

All lanes:

Beta Galactosidase at 0.05 µg

Secondary

All lanes:

HRP streptavidin conjugate at 1/40000 dilution

Predicted band size: 116 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

標識

Biotin

励起波長/蛍光波長

キャリアフリー

交差種

Escherichia coli

アプリケーション

Dot, IHC-P, ELISA, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/10000 - 1/50000", "ELISA-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Recombinant full length protein - Escherichia coli": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "" } } }

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製品の詳細

Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.

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出荷温度及び保存条件

製品の状態

Liquid

精製度

IgG fraction

精製方法

Ion exchange chromatography

精製に関する特記事項

Anti-BETA GALACTOSIDASE Antibody, Biotin Conjugated, is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.

バッファー組成

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Beta Galactosidase often called "β-gal" "b-galactosidase" or "b-gal" serves as an important enzyme that hydrolyzes β-galactosides into monosaccharides. It breaks down lactose into glucose and galactose which are easier to absorb. Beta-galactosidase expresses in many organisms such as E. coli where it is a well-known component of the lac operon. Its molecular weight in E. coli is approximately 116 kDa. The enzyme also appears in human tissues but is synthesized and regulated differently compared to bacterial systems.

Biological function summary

This enzyme is involved in the metabolism of galactose a critical aspect of cellular energy production. It can participate in various metabolic pathways converting complex carbohydrates into simpler sugars. In bacteria beta-galactosidase belongs to a protein complex controlled by the lac operon regulating lactose metabolism. In humans the enzyme takes part in lysosomal functions contributing to the degradation of glycoproteins and glycolipids therefore supporting normal cellular processes.

Pathways

Beta-galactosidase plays an important role in the lactose metabolism pathway. In E. coli it coheres with the enzymes of the lac system like lactose permease and transacetylase to facilitate lactose utilization. In human biology it participates in the lysosomal degradation pathway. Its function is associated with proteins such as galactosylceramidase which also break down galactolipids demonstrating its interaction in broader metabolic networks.

Deficiencies or malfunctions in beta-galactosidase lead to metabolic conditions like Galactosialidosis and Morquio syndrome Type B. These are lysosomal storage disorders caused by a lack of enzyme activity leading to accumulation of partially degraded macromolecules. The enzyme's impaired function connects with other proteins such as protective protein cathepsin A in the case of Galactosialidosis causing complex clinical symptoms through disrupted protein interactions and pathways.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information lacZ

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 7:12136 PubMed28939819

2017

In vivo target exploration of apidaecin based on Acquired Resistance induced by Gene Overexpression (ARGO assay).

Applications

Unspecified application

Species

Unspecified reactive species

Ken'ichiro Matsumoto,Kurato Yamazaki,Shun Kawakami,Daichi Miyoshi,Toshihiko Ooi,Shigeki Hashimoto,Seiichi Taguchi

Journal of neurotrauma 27:2067-76 PubMed20799882

2010

Sustained expression of vascular endothelial growth factor and angiopoietin-1 improves blood-spinal cord barrier integrity and functional recovery after spinal cord injury.

Applications

IHC-Fr

Species

Rat

Juan J Herrera,Laura M Sundberg,Lorena Zentilin,Mauro Giacca,Ponnada A Narayana

The Journal of comparative neurology 518:4675-701 PubMed20963823

2010

Target selection of proprioceptive and motor axon synapses on neonatal V1-derived Ia inhibitory interneurons and Renshaw cells.

Applications

Unspecified application

Species

Unspecified reactive species

Valerie C Siembab,Courtney A Smith,Laskaro Zagoraiou,Maria C Berrocal,George Z Mentis,Francisco J Alvarez

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 19:112-4 PubMed15486057

2004

Generation of purified oligodendrocyte progenitors from embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tamara Glaser,Alberto Perez-Bouza,Katja Klein,Oliver Brüstle

View all publications

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Abcam product promise

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Biotin Anti-beta Galactosidase 抗体

Biotin Anti-beta Galactosidase antibody

Western blot - Biotin Anti-beta Galactosidase antibody (AB6645)

Key facts

宿主種

クローン性

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise