Biotin Anti-bacterial Alkaline Phosphatase 抗体 (ab7321)
Key features and details
- Biotin Rabbit polyclonal to bacterial Alkaline Phosphatase
- Suitable for: WB, ELISA
- Reacts with: Escherichia coli
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-bacterial Alkaline Phosphatase antibody -
製品の詳細
Biotin Rabbit polyclonal to bacterial Alkaline Phosphatase -
由来種
Rabbit -
標識
Biotin -
アプリケーション
適用あり: WB, ELISAmore details -
種交差性
交差種: Escherichia coli -
免疫原
Full length native protein (purified) corresponding to Escherichia coli bacterial Alkaline Phosphatase.
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特記事項
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.424% Tripotassium orthophosphate -
Concentration information loading...
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精製度
Ion Exchange Chromatography -
特記事項(精製)
Anti-Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7321の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | ||
ELISA |
特記事項 |
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ELISA (1:4,000 to 1:20,000)
Immuno microscopy
Immunohistochemistry
as well as other peroxidase-antibody based enzymatic assays.
ターゲット情報
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関連性
Bacterial alkaline phosphatase (also known as PhoA) is derived from E. coli. It appears to require export to the periplasm to show enzymatic activity. Fusion of the secreted alkaline phosphatase to an integral cytoplasmic membrane protein of E. coli shows different activities depending on where with the membrane protein the alkaline phosphatase is fused. Consequently, analysis of alkaline phosphatase fusions to membrane proteins of unknown structure are generally useful in determining their membrane topologies. -
細胞内局在
Periplasm -
参照データベース
- Entrez Gene: 945041 Escherichia coli
- SwissProt: P00634 Escherichia coli
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別名
- Alkaline phosphatase antibody
- APase antibody
- BAP antibody
- PhoA antibody
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab7321 は 1 報の論文で使用されています。
- Alcaine SD et al. Bioengineering bacteriophages to enhance the sensitivity of phage amplification-based paper fluidic detection of bacteria. Biosens Bioelectron 82:14-9 (2016). PubMed: 27031186