Biotin Anti-Asparaginase 抗体 (ab34616)
Key features and details
- Biotin Rabbit polyclonal to Asparaginase
- Suitable for: WB, ELISA
- Reacts with: Escherichia coli
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-Asparaginase antibody -
製品の詳細
Biotin Rabbit polyclonal to Asparaginase -
由来種
Rabbit -
標識
Biotin -
アプリケーション
適用あり: WB, ELISAmore details -
種交差性
交差種: Escherichia coli -
免疫原
Asparaginase (Escherichia coli )
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading... -
精製度
Ion Exchange Chromatography -
特記事項（精製）
Anti-L-Asparaginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34616の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 37 kDa.
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| ELISA |
1/5000 - 1/25000.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 37 kDa. |
|
ELISA
1/5000 - 1/25000. |
ターゲット情報
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関連性
Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme. -
細胞内局在
Cytoplasmic, Secreted and Periplasm. -
参照データベース
- Entrez Gene: 1036607 Escherichia coli
- Entrez Gene: 1039460 Escherichia coli
- Entrez Gene: 946278 Escherichia coli
- Entrez Gene: 959650 Escherichia coli
- SwissProt: P00805 Escherichia coli
- SwissProt: P0A962 Escherichia coli
- SwissProt: P0A963 Escherichia coli
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別名
- AnsA antibody
- AnsB antibody
- Colaspase antibody
see all
画像
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Western blot - Biotin Anti-Asparaginase antibody (ab34616)Biotin Anti-Asparaginase antibody (ab34616) at 1/1000 dilution + L-asparaginase at 0.1 µg
Secondary
HRP-Streptavidin at 1/40000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab34616 は論文での使用が確認できていません。