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Anti-Biotin 抗体 [3E6] (ab36406)

Reviews (1)Q&A (1)References (3)

Key features and details

  • Mouse monoclonal [3E6] to Biotin
  • Suitable for: WB, ELISA
  • Reacts with: Species independent
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-Biotin antibody [3E6]
    Biotin 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [3E6] to Biotin

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, ELISAmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Chemical/ Small Molecule - biotinylated denatured Ovalbumin.

  • 特記事項


    Anti biotin antibodies can be used to detect both biotinylated proteins and nucleic acids.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab36406の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
Use at an assay dependent concentration.

In WB this antibody reacts with biotinylated proteins in both reduced and non-reduced forms.
ELISA
1/20000.

Strong reaction is seen in ELISA with biotin/ovalbumin coated onto the microtiter well and also in sandwich ELISA in combination with streptavidin.
特記事項
WB
Use at an assay dependent concentration.

In WB this antibody reacts with biotinylated proteins in both reduced and non-reduced forms.
ELISA
1/20000.

Strong reaction is seen in ELISA with biotin/ovalbumin coated onto the microtiter well and also in sandwich ELISA in combination with streptavidin.

ターゲット情報

  • 関連性

    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

  • 別名

    • Vitamin B4 antibody

参考文献 (3)

ab36406 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab36406 は 3 報の論文で使用されています。

  • Bailey EM  et al. Engineered IgG1-Fc Molecules Define Valency Control of Cell Surface Fc? Receptor Inhibition and Activation in Endosomes. Front Immunol 11:617767 (2020). PubMed: 33679705
  • Liu F  et al. Suppression of Membranous LRP5 Recycling, Wnt/ß-Catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P. Cell Rep 32:108049 (2020). PubMed: 32814052
  • Rosa AMM  et al. Colorimetric Detection of DNA Strands on Cellulose Microparticles Using ZZ-CBM Fusions and Gold Nanoparticles. Biotechnol J 14:e1800590 (2019). PubMed: 31144775

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Western blot abreview for Anti-Biotin antibody [212-01]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Human Recombinant protein (In Vitro Translation)
Loading amount
0.005 µg
Specification
In Vitro Translation
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 19 2008

Question

Dear Representative,

I am interested in buying few products from your company which i will be ordering frequently if satisfied.
1. Anti-biotin Antibody (ab36406)
2. Anti-RAd54B (ab57036)
are these antibodies glycosylated?

3. easy link FITC conjugation kit (ab102884)
4. easy linkcy5 conjugation kit (ab102876)
i observed that no purification is required after conjugating the labels to antibodies. what is the basis of such reliability? Does all labels bind to the Abs and there is no free label?

Please answer my highlighted doubts and send me an electronic quote for the below mentioned address giving any discount (if applicable).

Read More
Answer

Thank you for your inquiry and your interest in our products.

I will have to double-check with the lab regarding glycosylation of the antibodies since I do not have this information readily at hand. I will let you know once I have received this information.

For the EasyLink kits, there is a quenching step which removes any excess label and reduces background. You can find more information about the labeling process here: https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink

Additional FAQs regarding these kits can be found here: https://www.abcam.com/index.html?pageconfig=resource&rid=13156

I will email you a quote for the 4 products. Currently we are running a promotion where if you buy any primary, you can get a RabMAb for free! Just mention the code RABMAB-XBSMG on the order and the cost of the rabbit monoclonal antibody will be removed from the order.

I hope this information helps. I will be in touch again with more information regarding the antibodies you requested.

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