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AB7338

Biotin Anti-alpha 2 Macroglobulin 抗体

Biotin Anti-alpha 2 Macroglobulin antibody

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(1 Publication)

Goat Polyclonal alpha 2 Macroglobulin antibody - conjugated to Biotin. Suitable for WB, ELISA and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Human A2M.

別名を表示する

CPAMD5, FWP007, A2M, Alpha-2-macroglobulin, Alpha-2-M, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5

Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

標識

Biotin

励起波長/蛍光波長
キャリアフリー

No

交差種

Human

アプリケーション

ELISA, WB

applications

免疫原

Native Full Length Protein corresponding to Human A2M.

P01023

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/20000 - 1/100000", "ELISA-species-notes": "<p></p>" } } }
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製品の詳細

Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.
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出荷温度及び保存条件

製品の状態
Liquid
精製度
IgG fraction
精製方法
Ion exchange chromatography
精製に関する特記事項
Anti-Alpha-2-Macroglobulin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
バッファー組成
Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-2-macroglobulin also known as alpha-2-M and A2M is a large glycoprotein with a molecular mass of approximately 725 kDa. It is expressed in the liver and is found in high concentrations in the blood plasma. This protein functions mechanically as a broad-spectrum protease inhibitor. It can trap proteases within its structure preventing them from accessing substrates. Additionally alpha-2-macroglobulin can bind to cytokines and growth factors altering their availability and activities.
Biological function summary

Alpha-2-macroglobulin plays an important role in maintaining homeostasis by regulating protease activities and modulating immune system reactions. It does not form a permanent complex but can transiently associate with the proteases and various molecules mentioned. By interacting with proteases it controls processes like coagulation and fibrinolysis and participates in the clearance of proteinases and growth factors from the circulation. It influences cell signaling pathways and inflammatory responses highlighting its significance in maintaining balance within the body.

Pathways

Alpha-2-macroglobulin is an important component in the immune response and coagulation pathways. In the complement system it binds with complement components to regulate their activities. It associates with proteins such as plasmin and thrombin which are important for blood clotting and fibrinolysis. These relationships emphasize the protein's involvement in both protective mechanisms against pathogens and the regulation of blood coagulation and breakdown connecting the immune and hemostatic systems.

Alpha-2-macroglobulin has been linked to Alzheimer's disease and liver diseases. In Alzheimer's altered levels of this protein might affect the amyloid-beta peptide clearance due to its protease inhibition and binding properties. It associates with proteins like lipoprotein receptor-related protein-1 (LRP1) influencing amyloid-beta metabolism. In liver diseases its levels can indicate liver dysfunction or inflammation given its synthesis in the liver and can relate to proteins involved in inflammation such as cytokines. Its modulation of protease activity makes it a critical target in understanding these disorders.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
See full target information A2M
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Biochimica et biophysica acta 1690:203-7 PubMed15511627

2004

Significantly increased fractions of transformed to total alpha2-macroglobulin concentrations in plasma from patients with multiple sclerosis.

Applications

ELISA

Species

Unspecified reactive species

Poul Erik H Jensen,Signe Humle Jørgensen,Pameli Datta,Per Soelberg Sørensen
View all publications
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代替製品

Primary Antibodies

AB181656

Anti-alpha 2 Macroglobulin antibody

Western blot - Anti-alpha 2 Macroglobulin antibody (AB181656)

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Primary Antibodies

AB109422

Anti-alpha 2 Macroglobulin antibody [EPR4432]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 2 Macroglobulin antibody [EPR4432] (AB109422)

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Primary Antibodies

AB156015

Anti-alpha 2 Macroglobulin antibody [EPSISR39]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-alpha 2 Macroglobulin antibody [EPSISR39] (AB156015)

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Primary Antibodies

AB133299

Anti-alpha 2 Macroglobulin antibody [EPR4431]

RabMAb|Recombinant

Western blot - Anti-alpha 2 Macroglobulin antibody [EPR4431] (AB133299)

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組合せ製品

Assay Kits

AB108888

Human alpha 2 Macroglobulin ELISA Kit

Competitive ELISA - Human alpha 2 Macroglobulin ELISA Kit (AB108888)

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  • 0
Primary Antibodies

AB195045

Anti-PAX6 antibody [EPR15858]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [EPR15858] (AB195045)

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  • 9
Primary Antibodies

AB46799

Anti-alpha 1 Fetoprotein antibody

Western blot - Anti-alpha 1 Fetoprotein antibody (AB46799)

  • 4
  • 7
Primary Antibodies

AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

  • 5
  • 1
Assay Kits

AB108888

Human alpha 2 Macroglobulin ELISA Kit

Competitive ELISA - Human alpha 2 Macroglobulin ELISA Kit (AB108888)

  • 0
  • 0

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