Anti-Bile Acid Receptor NR1H4 抗体 (ab85606)
Key features and details
- Rabbit polyclonal to Bile Acid Receptor NR1H4
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Bile Acid Receptor NR1H4 antibody
Bile Acid Receptor NR1H4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Bile Acid Receptor NR1H4 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Hamster, Cow
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免疫原
Synthetic peptide corresponding to Bile Acid Receptor NR1H4 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab97929) -
ポジティブ･コントロール
- This antibody gave a positive signal in the following lysates: HepG2 Whole Cell; Human Kidney Tissue; Human Small Intestine.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98.98% PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85606の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
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| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa).
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa). |
ターゲット情報
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機能
Ligand-activated transcription factor. Receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. Represses the transcription of the cholesterol 7-alpha-hydroxylase gene (CYP7A1) through the induction of NR0B2 or FGF19 expression, via two distinct mechanisms. Activates the intestinal bile acid-binding protein (IBABP). Activates the transcription of bile salt export pump ABCB11 by directly recruiting histone methyltransferase CARM1 to this locus. -
配列類似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 9971 Human
- Entrez Gene: 20186 Mouse
- Omim: 603826 Human
- SwissProt: Q3SZL0 Cow
- SwissProt: Q96RI1 Human
- SwissProt: Q60641 Mouse
- Unigene: 282735 Human
- Unigene: 3095 Mouse
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別名
- BAR antibody
- Bile acid receptor antibody
- Farnesoid X activated receptor antibody
see all
画像
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Western blot - Anti-Bile Acid Receptor NR1H4 antibody (ab85606)All lanes : Anti-Bile Acid Receptor NR1H4 antibody (ab85606) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lane 3 : Human small intestine tissue lysate - total protein (ab29276)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Bile Acid Receptor NR1H4 antibody (ab85606)ICC/IF image of ab85606 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85606, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa and HepG2 cells at 5µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab85606 は 4 報の論文で使用されています。
- Liu F et al. Nigakinone alleviates DSS-induced experimental colitis via regulating bile acid profile and FXR/NLRP3 signaling pathways. Phytother Res 37:15-34 (2023). PubMed: 36054406
- Jia Y et al. Activation of FXR by ganoderic acid A promotes remyelination in multiple sclerosis via anti-inflammation and regeneration mechanism. Biochem Pharmacol 185:114422 (2021). PubMed: 33482151
- Jia Y et al. Ganoderic Acid A Attenuates LPS-Induced Neuroinflammation in BV2 Microglia by Activating Farnesoid X Receptor. Neurochem Res 46:1725-1736 (2021). PubMed: 33821438
- Preidis GA et al. Coagulopathy in Malnourished Mice Is Sexually Dimorphic and Regulated by Nutrient-Sensing Nuclear Receptors. Hepatol Commun 4:1835-1850 (2020). PubMed: 33305154