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Anti-BIII 抗体 (ab66426)

Reviews (1)Q&A (2)References (1)

Key features and details

  • Rabbit polyclonal to BIII
  • Suitable for: ELISA, WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-BIII antibody
    BIII 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to BIII

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ELISA, WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab66426の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
WB (1)
1/500 - 1/5000. Predicted molecular weight: 262 kDa.
IF
1/100 - 1/500.
特記事項
ELISA
Use at an assay dependent concentration.
WB
1/500 - 1/5000. Predicted molecular weight: 262 kDa.
IF
1/100 - 1/500.

ターゲット情報

  • 機能

    Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1B gives rise to N-type calcium currents. N-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by omega-conotoxin-GVIA (omega-CTx-GVIA) and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to dihydropyridines (DHP), and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing alpha-1B subunit may play a role in directed migration of immature neurons.

  • 組織特異性

    Isoform Alpha-1b-1 and isoform Alpha-1b-2 are expressed in the central nervous system, but not in skeletal muscle or aorta.

  • 関連疾患

    Dystonia 23

  • 配列類似性

    Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1B subfamily.
    Contains 1 EF-hand domain.

  • ドメイン

    Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.

  • 翻訳後修飾

    Phosphorylated in vitro by CaM-kinase II, PKA, PKC and CGPK.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • alpha-1 polypeptide isoform 5 antibody
    • BIII antibody
    • Brain calcium channel III antibody
    • CAC1B_HUMAN antibody
    • CACH5 antibody
    • CACNA1B (N type) antibody
    • CACNA1B antibody
    • CACNL1A5 antibody
    • CACNN antibody
    • Calcium channel alpha12.2 subunit antibody
    • Calcium channel antibody
    • Calcium channel L type alpha 1 polypeptide isoform 5 antibody
    • Calcium channel L type, alpha 1 polypeptide antibody
    • Calcium channel N type antibody
    • Calcium channel voltage dependent alpha 1B subunit N type antibody
    • Calcium channel voltage dependent L type alpha 1B subunit antibody
    • Calcium channel voltage dependent N type alpha 1B subunit antibody
    • Cav2.2 antibody
    • Cav2.2 voltage gated Ca2+ channel antibody
    • L type antibody
    • Voltage dependent N type calcium channel subunit alpha 1B antibody
    • Voltage gated calcium channel alpha subunit Cav2.2 antibody
    • Voltage-dependent N-type calcium channel subunit alpha-1B antibody
    • Voltage-gated calcium channel subunit alpha Cav2.2 antibody
    see all

参考文献 (1)

ab66426 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab66426 は 1 報の論文で使用されています。

  • Barzan R  et al. N- and L-Type Voltage-Gated Calcium Channels Mediate Fast Calcium Transients in Axonal Shafts of Mouse Peripheral Nerve. Front Cell Neurosci 10:135 (2016). PubMed: 27313508

レビューと Q&A

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レビューを送る 質問を送る

1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-BIII antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (spinal cord)
Gel Running Conditions
Reduced Denaturing (8-12%)
Loading amount
50 µg
Specification
spinal cord
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Alexandre Magno

Verified customer

投稿 Aug 14 2018

Question

I would like to do immunhistochemistry with some antibody against CACNA1B using mouse sections. There are 2 different antibodies predicted to react to mouse samples for immunostaining in your company (ab58551, ab66426) . The ab58551 seems to be same antibodies available from many other companies, and it did not work very well with my samples. Therefore, I would like to try other antibody (ab66426), but no immunogen information except for it was from human. I would appreciate it if you could provide me the immunogen information and how much percentage of the identity/homology of the immunogen between human and mouse. Thank you.

Read More
Answer

Thank you very much for your interest in ab66426. To our knowledge, ab66426 has not been tested in mouse, however the peptide used to develop ab66426 is 100% homologous with murine protein. Therefore, I can offer a discount off a future purchase if you buy ab66426 now, test it in mouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab66426 in mouse. I will then send a discount code. This code must be issued before purchasing ab66426 so please wait for my reply before ordering. 2. Purchase ab66426 either by phone, fax, or online (www.abcam.com). 3. Test it in mouse. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab66426 turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Question

I'm interested to find out if this anti-CACNA1b antibody is able to be used in zebrafish embryos for immunohistochemistry. I would appreciate if you could provide sequence details on the immunoreactive epitope used since it is only stated as "N terminal domain of human CACNA1B (N type)" on the website.

Read More
Answer

Thank you for your enquiry. Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is considered commercially sensitive. We aim to provide as much information as possible to customers and I am sorry this has not been possible on this occasion. Nevertheless, I can confirm the polyclonal antibody ab66426 doesn't react in Zebrafish. Should you have any further questions, please do not hesitate to contact us.

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