Anti-BIG1 抗体 [EPR10046(2)] (ab183747)
![Western blot - Anti-BIG1 antibody [EPR10046(2)] (ab183747)](https://www.abcam.co.jp/ps/products/183/ab183747/Images/ab183747-215830-anti-big1-antibody-epr100462-western-blot.jpg "Western blot - Anti-BIG1 antibody [EPR10046(2)] (ab183747)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10046(2)] to BIG1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-BIG1 antibody [EPR10046(2)]
BIG1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10046(2)] to BIG1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Jurkat, 293T and A431 cell lysates; Human kidney tissue; 293T and A431 cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR10046(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab183747の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/2000. Detects a band of approximately 250 kDa (predicted molecular weight: 209 kDa).
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| ICC/IF |
1/100.
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| 特記事項 |
|---|
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WB
1/1000 - 1/2000. Detects a band of approximately 250 kDa (predicted molecular weight: 209 kDa). |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ICC/IF
1/100. |
ターゲット情報
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関連性
BIG1 is a brefeldin A-inhibited guanine nucleotide exchange protein (GEP) that activates ADP-ribosylation factor (ARF) GTPases and functions in vesicle formation from Golgi membranes. -
細胞内局在
Cytoplasmic, Golgi Apparatus and Nuclear -
参照データベース
- Entrez Gene: 10565 Human
- Entrez Gene: 211673 Mouse
- Entrez Gene: 312915 Rat
- Omim: 604141 Human
- SwissProt: Q9Y6D6 Human
- SwissProt: G3X9K3 Mouse
- SwissProt: D4A631 Rat
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別名
- ADP ribosylation factor guanine nucleotide exchange factor 1(brefeldin A inhibited) antibody
- ADP-ribosylation factor guanine nucleotide exchange factor 1 antibody
- ARF GEP1 antibody
see all
画像
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Western blot - Anti-BIG1 antibody [EPR10046(2)] (ab183747)All lanes : Anti-BIG1 antibody [EPR10046(2)] (ab183747) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 293T cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 209 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG1 antibody [EPR10046(2)] (ab183747)](https://www.abcam.co.jp/ps/products/183/ab183747/Images/ab183747-215829-anti-big1-antibody-epr100462-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG1 antibody [EPR10046(2)] (ab183747)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling BIG1 with ab183747 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)](https://www.abcam.co.jp/ps/products/183/ab183747/Images/ab183747-215828-anti-big1-antibody-epr100462-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling BIG1 with ab183747 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)](https://www.abcam.co.jp/ps/products/183/ab183747/Images/ab183747-215827-anti-big1-antibody-epr100462-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling BIG1 with ab183747 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
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![Anti-BIG1 antibody [EPR10046(2)] (ab183747)](https://www.abcam.co.jp/ps/products/183/ab183747/Images/ab183747-5-benefits-of-recombinant-antibodies.png)
Anti-BIG1 antibody [EPR10046(2)] (ab183747)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (2)
ab183747 は 2 報の論文で使用されています。
- Yue X et al. Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner. Commun Biol 4:1370 (2021). PubMed: 34876695
- Han J et al. miR-215 suppresses papillary thyroid cancer proliferation, migration, and invasion through the AKT/GSK-3ß/Snail signaling by targeting ARFGEF1. Cell Death Dis 10:195 (2019). PubMed: 30814512