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RabMAb

Anti-beta Synuclein 抗体 [EP1537Y] (ab76111)

Submit a review Submit a question References (12)
Western blot - Anti-beta Synuclein antibody [EP1537Y] (ab76111)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] (ab76111)
  • Western blot - Anti-beta Synuclein antibody [EP1537Y] (ab76111)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Synuclein antibody [EP1537Y] (ab76111)
  • Flow Cytometry (Intracellular) - Anti-beta Synuclein antibody [EP1537Y] (ab76111)
  • Immunoprecipitation - Anti-beta Synuclein antibody [EP1537Y] (ab76111)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] (ab76111)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] (ab76111)

Key features and details

  • Rabbit monoclonal [EP1537Y] to beta Synuclein
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human, Recombinant fragment
  • Isotype: IgG

Conjugates logo Related conjugates and formulations

Carrier Free HRP

製品の概要

  • 製品名

    Anti-beta Synuclein antibody [EP1537Y]
    beta Synuclein 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP1537Y] to beta Synuclein

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat, Human, Recombinant fragment

  • 免疫原

    Synthetic peptide within Human beta Synuclein aa 50-150. The exact sequence is proprietary.
    Database link: Q16143

  • ポジティブ･コントロール

    • WB: Human, mouse, and rat brain tissue lysate; IP: Human brain lysate; Flow Cyt (intra): PC-12 cells; ICC/IF: PC-12 cells; IHC: Human, mouse and rat cerebellum tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab76111の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/100.
WB
1/5000 - 1/20000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).
IP
1/20.

For unpurified use at 1/40
IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/100-1/250 dilution
ICC/IF
1/100.

For unpurified use at 1/100-1/250 dilution.
特記事項
Flow Cyt (Intra)
1/100.
WB
1/5000 - 1/20000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).
IP
1/20.

For unpurified use at 1/40
IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/100-1/250 dilution
ICC/IF
1/100.

For unpurified use at 1/100-1/250 dilution.

ターゲット情報

  • 機能

    Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease.

  • 組織特異性

    Expressed predominantly in brain; concentrated in presynaptic nerve terminals.

  • 配列類似性

    Belongs to the synuclein family.

  • 翻訳後修飾

    Phosphorylated. Phosphorylation by G-protein coupled receptor kinases (GRK) is more efficient than phosphorylation by CK1, CK2 and CaM-kinase II.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 14 kDa brain-specific protein antibody
    • Beta-synuclein antibody
    • Phosphoneuroprotein 14 antibody
    • PNP14 antibody
    • Sncb antibody
    • Synuclein beta antibody
    • SYUB_HUMAN antibody
    see all

画像

  • Western blot - Anti-beta Synuclein antibody [EP1537Y] (ab76111)
    Western blot - Anti-beta Synuclein antibody [EP1537Y] (ab76111)
    All lanes : Anti-beta Synuclein antibody [EP1537Y] (ab76111) at 1/10000 dilution (purified)

    Lane 1 : Human brain lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 14 kDa
    Observed band size: 18 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] (ab76111)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] (ab76111)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling beta Synuclein with purified ab76111 at 1/50 dilution (2.4 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-beta Synuclein antibody [EP1537Y] (ab76111)
    Western blot - Anti-beta Synuclein antibody [EP1537Y] (ab76111)
    All lanes : Anti-beta Synuclein antibody [EP1537Y] (ab76111) at 1/10000 dilution (purified)

    Lane 1 : His-Tagged human beta Synuclein (aa 1 to 134) recombinant protein
    Lane 2 : His-Tagged alpha synuclein (aa 1 to 140) recombinant protein

    Lysates/proteins at 0.015 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 14 kDa
    Observed band size: 18 kDa why is the actual band size different from the predicted?



    A: Anti-beta Synuclein antibody [EP1537Y] (ab76111)

    B: Anti-Alpha-synuclein antibody [MJFR1] (ab138501) 

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Synuclein antibody [EP1537Y] (ab76111)
    Immunocytochemistry/ Immunofluorescence - Anti-beta Synuclein antibody [EP1537Y] (ab76111)

    Immunocytochemistry/ Immunofluorescence analysis of PC-12 (Rat adrenal gland pheochromocytoma) cells labeling beta Synuclein with purified ab76111 at 1/50 dilution (2.4 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-beta Synuclein antibody [EP1537Y] (ab76111)
    Flow Cytometry (Intracellular) - Anti-beta Synuclein antibody [EP1537Y] (ab76111)

    Intracellular Flow Cytometry analysis of PC-12 (Rat adrenal gland pheochromocytoma) cells labeling beta Synuclein with purified ab76111 at 1/100 dilution (10µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

     

     

  • Immunoprecipitation - Anti-beta Synuclein antibody [EP1537Y] (ab76111)
    Immunoprecipitation - Anti-beta Synuclein antibody [EP1537Y] (ab76111)
    Purified ab76111 at 1/20 dilution (0.6 µg) immunoprecipitating beta Synuclein in Human brain lysate.
    Lane 1 (input): Human brain lysate 10 µg
    Lane 2 (+): ab76111 + Human brain lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76111 in Human brain lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 18 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] (ab76111)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] (ab76111)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling beta Synuclein with purified ab76111 at 1/50 dilution (2.4 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] (ab76111)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody [EP1537Y] (ab76111)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling beta Synuclein with purified ab76111 at 1/50 dilution (2.4 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

参考文献 (12)

ab76111 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab76111 は 12 報の論文で使用されています。

  • Dias SB  et al. Age-Related Changes of the Synucleins Profile in the Mouse Retina. Biomolecules 13:N/A (2023). PubMed: 36671565
  • Brown JL  et al. SNCA genetic lowering reveals differential cognitive function of alpha-synuclein dependent on sex. Acta Neuropathol Commun 10:180 (2022). PubMed: 36517890
  • Evans T  et al. Visualization and Measurement of Multiple Components of the Autophagy Flux. Methods Mol Biol 1854:1-12 (2019). PubMed: 29936691
  • Kobayashi J  et al. Extracellular a-synuclein enters dopaminergic cells by modulating flotillin-1-assisted dopamine transporter endocytosis. FASEB J 33:10240-10256 (2019). PubMed: 31211923
  • Benskey MJ  et al. Silencing Alpha Synuclein in Mature Nigral Neurons Results in Rapid Neuroinflammation and Subsequent Toxicity. Front Mol Neurosci 11:36 (2018). PubMed: 29497361
View all Publications for this product

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