AB6165

Anti-beta Synuclein 抗体

Name: Anti-beta Synuclein 抗体 (ab6165) | Abcam
Brand: Abcam Limited
SKU: AB6165
Price: 78000 JPY
Availability: InStock
Rating: 4 (1 reviews)

Anti-beta Synuclein antibody

(1 Review)

(8 Publications)

Rabbit Polyclonal beta Synuclein antibody. Suitable for IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human SNCB aa 100 to C-terminus.

別名を表示する

Beta-synuclein, SNCB

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody (AB6165)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody (AB6165)

ab6165 staining beta synuclein in human occipital cortex.
Left panel : with primary antibody diluted 1 : 4000. Right panel : isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature : sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P

applications

免疫原

Synthetic Peptide within Human SNCB aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q16143

特異性

Less than 0.1% cross reactivity against human alpha-synuclein Cross reactivity: This antibody is known to react with human and rat. Not yet tested in other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製度

Whole antiserum

バッファー組成

pH: 7 - 7.4

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Add glycerol to a final volume of 50% for extra stability and aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Beta-synuclein also known as β-synuclein or SNCB is a protein that plays a role in the nervous system. It is a small protein with a molecular weight around 14 kDa. Predominantly beta-synuclein is found in presynaptic terminals of neurons in the central nervous system including the brain and spinal cord. This protein shares significant amino acid sequence identity with alpha-synuclein and gamma-synuclein comprising the synuclein family. It acts as a modulator of neuronal and synaptic function influencing the dynamics of neurotransmitter release.

Biological function summary

Beta-synuclein contributes to cellular homeostasis and synaptic integrity. It is not typically found as part of a larger protein complex like its relative alpha-synuclein which forms toxic aggregates. Instead beta-synuclein exhibits neuroprotective properties helping maintain neuronal health. It possibly regulates synaptic vesicle cycling and mitigates levels of alpha-synuclein aggregation. Research suggests that loss of beta-synuclein function might disrupt synaptic stability leading to neurological issues.

Pathways

Beta-synuclein engages in synaptic function and plasticity. It participates in pathways related to neurotransmitter release and synaptic vesicle trafficking. Beta-synuclein is connected to calcium/calmodulin-dependent protein kinase II (CaMKII) pathways. This interaction supports beta-synuclein's regulatory impact on synaptic vesicle dynamics negatively modulating the aggregation-prone behaviors seen in alpha-synuclein during synucleinopathies.

Beta-synuclein plays a role in neurodegenerative diseases like Parkinson’s disease and dementia with Lewy bodies. Research highlights its relationship with these conditions due to its interaction with alpha-synuclein known to form pathological aggregates and contribute to disease progression. Beta-synuclein's modulation of alpha-synuclein activity can impact the development and progression of these disorders. There is ongoing interest in its potential protective role suggesting it might be leveraged in therapeutic strategies against synucleinopathy-related diseases.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease.

See full target information SNCB

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

NPJ Parkinson's disease 10:1 PubMed38167744

2024

Aggregate-prone brain regions in Parkinson's disease are rich in unique N-terminus α-synuclein conformers with high proteolysis susceptibility.

Applications

Unspecified application

Species

Unspecified reactive species

James A Wiseman,Helen C Murray,Richard L M F Faull,Michael Dragunow,Clinton P Turner,Birger Victor Dieriks,Maurice A Curtis

Cells 12: PubMed36831238

2023

Interneuronal In Vivo Transfer of Synaptic Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Klinkenberg,Michael Helwig,Rita Pinto-Costa,Angela Rollar,Raffaella Rusconi,Donato A Di Monte,Ayse Ulusoy

Disease models & mechanisms 10:619-631 PubMed28108469

2017

Blood RNA biomarkers in prodromal PARK4 and rapid eye movement sleep behavior disorder show role of complexin 1 loss for risk of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Suna Lahut,Suzana Gispert,Özgür Ömür,Candan Depboylu,Kay Seidel,Jorge Antolio Domínguez-Bautista,Nadine Brehm,Hülya Tireli,Karl Hackmann,Caroline Pirkevi,Barbara Leube,Vincent Ries,Kerstin Reim,Nils Brose,Wilfred F den Dunnen,Madrid Johnson,Zsuzsanna Wolf,Marc Schindewolf,Wiebke Schrempf,Kathrin Reetz,Peter Young,David Vadasz,Achilleas S Frangakis,Evelin Schröck,Helmuth Steinmetz,Marina Jendrach,Udo Rüb,Ayşe Nazlı Başak,Wolfgang Oertel,Georg Auburger

Journal of neuroscience research 94:717-35 PubMed27265751

2016

LRRK2 knockdown in zebrafish causes developmental defects, neuronal loss, and synuclein aggregation.

Applications

Unspecified application

Species

Unspecified reactive species

Shubhangi Prabhudesai,Fatima Zahra Bensabeur,Rashed Abdullah,Indranil Basak,Solange Baez,Guido Alves,Nathalia G Holtzman,Jan Petter Larsen,Simon Geir Møller

Brain : a journal of neurology 139:856-70 PubMed26719384

2016

Brain propagation of transduced α-synuclein involves non-fibrillar protein species and is enhanced in α-synuclein null mice.

Applications

Species

Mouse

Michael Helwig,Michael Klinkenberg,Raffaella Rusconi,Ruth E Musgrove,Nour K Majbour,Omar M A El-Agnaf,Ayse Ulusoy,Donato A Di Monte

Neuropathology and applied neurobiology 38:737-43 PubMed22686686

2012

Pick's pathology in Parkinson's disease with dementia.

Applications

Unspecified application

Species

Unspecified reactive species

D Vilas,M J Marti,T Botta-Orfila,M Colom-Cadena,E Gelpi

Brain : a journal of neurology 133:3724-33 PubMed20959308

2010

The decrease of β-synuclein in cortical brain areas defines a molecular subgroup of dementia with Lewy bodies.

Applications

Species

Human

Katrin Beyer,Montserrat Domingo-Sàbat,Cristina Santos,Eduardo Tolosa,Isidro Ferrer,Aurelio Ariza

The Journal of biological chemistry 276:45320-9 PubMed11560921

2001

alpha-Synuclein affects the MAPK pathway and accelerates cell death.

Applications

Species

Human

A Iwata,M Maruyama,I Kanazawa,N Nukina

View all publications

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Abcam product promise

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Anti-beta Synuclein 抗体

Anti-beta Synuclein antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody (AB6165)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

出荷温度及び保存条件

製品の状態

精製度

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (8)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise