Anti-Beta Lactamase 抗体 [8A5.A10] (ab12251)
Key features and details
- Mouse monoclonal [8A5.A10] to Beta Lactamase
- Suitable for: ELISA, WB
- Reacts with: Escherichia coli
- Isotype: IgG1
製品の概要
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製品名
Anti-Beta Lactamase antibody [8A5.A10] -
製品の詳細
Mouse monoclonal [8A5.A10] to Beta Lactamase -
由来種
Mouse -
特異性
This antibody specifically recognizes TEM type beta lactamases. -
アプリケーション
適用あり: ELISA, WBmore details -
種交差性
交差種: Escherichia coli -
免疫原
Recombinant full length protein corresponding to Mouse Beta Lactamase.
Database link: P62593 -
特記事項
Dilute in PBS or medium which is identical to that used in the assay system.
This product was changed from ascites to tissue culture supernatant on 19/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from TCS -
ポリ/モノ
モノクローナル -
クローン名
8A5.A10 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12251の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use a concentration of 10 - 20 µg/ml.
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WB |
Use a concentration of 10 µg/ml. Predicted molecular weight: 31.5 kDa.
(31.5kDa is the molecular weight of the unprocessed precursor.) |
特記事項 |
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ELISA
Use a concentration of 10 - 20 µg/ml. |
WB
Use a concentration of 10 µg/ml. Predicted molecular weight: 31.5 kDa. (31.5kDa is the molecular weight of the unprocessed precursor.) |
ターゲット情報
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関連性
The beta lactam antibiotics (penicillins and cephalosporins) are the most frequently used antimicrobial agents. All of the beta lactams are structurally related through the presence of a core beta lactam ring. Bacterial resistance to beta lactams continues to increase, primarily due to the production of beta lactamases. Beta lactamases catalyze the hydrolysis of the beta lactam bond, which destroys antibacterial activity. Bacteria that produce TEM type or SHV type beta lactamases have point mutations in structural genes that have extended the substrate specificity of these beta lactamases. As a result, many of the beta lactamase producing Gram negative pathogens have become multidrug resistant. -
別名
- AmpA antibody
- AmpC antibody
- Cephalosporinase antibody
データシートおよび資料
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Datasheet download
参考文献 (31)
ab12251 は 31 報の論文で使用されています。
- Shi Y et al. Legionella longbeachae Regulates the Association of Polyubiquitinated Proteins on Bacterial Phagosome with Multiple Deubiquitinases. Microbiol Spectr 11:e0417922 (2023). PubMed: 36790208
- Shi Y et al. Legionella longbeachae effector protein RavZ inhibits autophagy and regulates phagosome ubiquitination during infection. PLoS One 18:e0281587 (2023). PubMed: 36758031
- Chaoprasid P et al. Crystal structure of bacterial cytotoxic necrotizing factor CNFY reveals molecular building blocks for intoxication. EMBO J 40:e105202 (2021). PubMed: 33410511
- Bielaszewska M et al. In Vivo Secretion of ß-Lactamase-Carrying Outer Membrane Vesicles as a Mechanism of ß-Lactam Therapy Failure. Membranes (Basel) 11:N/A (2021). PubMed: 34832035
- Naha A et al. Deciphering the possible role of ctxB7 allele on higher production of cholera toxin by Haitian variant Vibrio cholerae O1. PLoS Negl Trop Dis 14:e0008128 (2020). PubMed: 32236098