Anti-beta III Tubulin 抗体 (ab41489)
Key features and details
- Chicken polyclonal to beta III Tubulin
- Suitable for: ICC/IF
- Reacts with: Mouse
- Isotype: IgY
製品の概要
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製品名
Anti-beta III Tubulin antibody
beta III Tubulin 一次抗体 製品一覧 -
製品の詳細
Chicken polyclonal to beta III Tubulin -
由来種
Chicken -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Mouse -
免疫原
Three different synthetic peptides conjugated to KLH. These synthetic peptides corresponded to different regions of the beta III Tubulin gene product, but are shared between human and rat.
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特記事項
Equal volumes of three different affinity-purified anti-peptide antibodies were combined to make this product.
Do not freeze antibody unless you want to store for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7
Preservative: 0.02% Sodium azide
Constituent: 99.98% 1X PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgY -
研究分野
関連製品
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab41489の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (3) |
1/1000 - 1/2000.
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| 特記事項 |
|---|
|
ICC/IF
1/1000 - 1/2000. |
ターゲット情報
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機能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. -
組織特異性
Expression is primarily restricted to central and peripheral nervous system. -
関連疾患
Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy. -
配列類似性
Belongs to the tubulin family. -
ドメイン
The highly acidic C-terminal region may bind cations such as calcium. -
翻訳後修飾
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 22152 Mouse
- SwissProt: Q9ERD7 Mouse
- Unigene: 40068 Mouse
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別名
- beta 3 tubulin antibody
- beta 4 antibody
- beta-4 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody (ab41489)
Immunocytochemistry/Immunofluorescence analysis of SpH21 cells in culture labelling beta III Tubulin (green) with ab41489. Cells were fixed with 4% paraformaldehyde. Red - Rabbit anti-GAD67, Blue - DAPI.
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Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody (ab41489)Immunocytochemistry/Immunofluorescence analysis of SpH64 cells in culture labelling beta III Tubulin (green) with ab41489. Cells were fixed with 4% paraformaldehyde. Red - Rabbit anti-GFP, Blue: DAPI.
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Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody (ab41489)Image from Ohmine S et al., Stem Cell Research & Therapy 2011, 2:46, 16 November 2011. Fig 5.; doi: 10.1186/scrt87.Immunofluorescence analysis of HPC and PBMC blood-derived iPSC clones, staining beta III Tubulin (green) with ab41489.
Cells were fixed with 4% PFA and blocked for 30 minutes in PBS with 5% fetal bovine serum. Cells were incubated with primary antibody overnight at 4°C. A FITC-conjugated donkey anti-chicken IgG was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (66)
ab41489 は 66 報の論文で使用されています。
- Lang Q et al. In vivo imaging of axonal transport in peripheral nerves of rodent forelimbs. Neuronal Signal 7:NS20220098 (2023). PubMed: 36743438
- Cervetto C et al. Assessment of neurotransmitter release in human iPSC-derived neuronal/glial cells: a missing in vitro assay for regulatory developmental neurotoxicity testing. Reprod Toxicol 117:108358 (2023). PubMed: 36863571
- Marton S et al. SOD1G93A Astrocyte-Derived Extracellular Vesicles Induce Motor Neuron Death by a miRNA-155-5p-Mediated Mechanism. ASN Neuro 15:17590914231197527 (2023). PubMed: 37644868
- Smith AS et al. High-content analysis and Kinetic Image Cytometry identify toxicity and epigenetic effects of HIV antiretrovirals on human iPSC-neurons and primary neural precursor cells. J Pharmacol Toxicol Methods 114:107157 (2022). PubMed: 35143957
- Azzarelli R et al. ASCL1 phosphorylation and ID2 upregulation are roadblocks to glioblastoma stem cell differentiation. Sci Rep 12:2341 (2022). PubMed: 35149717