Anti-beta II Tubulin 抗体 [7B9] (ab28035)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [7B9] to beta II Tubulin
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-beta II Tubulin antibody [7B9]
beta II Tubulin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [7B9] to beta II Tubulin -
由来種
Mouse -
アプリケーション
適用あり: WB, IP, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human, mouse and rat brain tissue lysate. U-87 MG, C6, PC-12, Y79 and MDA-MB-231 whole cell lysate. ICC/IF: U-87 MG, Neuro-2a and PC-12 cells. IP: Neuro-2a whole cell lysate.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
7B9 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28035の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000. Predicted molecular weight: 50 kDa.
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IP |
1/30.
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ICC/IF |
1/50.
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特記事項 |
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WB
1/1000. Predicted molecular weight: 50 kDa. |
IP
1/30. |
ICC/IF
1/50. |
ターゲット情報
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機能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the tubulin family. -
ドメイン
The highly acidic C-terminal region may bind cations such as calcium. -
翻訳後修飾
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 10383 Human
- Entrez Gene: 347733 Human
- Entrez Gene: 7280 Human
- Entrez Gene: 22151 Mouse
- Entrez Gene: 227613 Mouse
- Entrez Gene: 73710 Mouse
- Entrez Gene: 291081 Rat
- Entrez Gene: 296554 Rat
see all -
別名
- bA506K6.1 antibody
- beta 2 tubulin antibody
- Beta2 antibody
see all
画像
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All lanes : Anti-beta II Tubulin antibody [7B9] (ab28035) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 50 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
Negative control: Human heart (PMID:20191564).
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG cells labelling beta II Tubulin with ab28035 at 1/50 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in U-87 MG cell line. ab179513 anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab28035 at a 1/500 dilution followed by ab150080 at a 1/500 dilution.
Negative control 2: ab179513 at a 1/500 dilution followed by ab150113 at a 1/1000 dilution.
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All lanes : Anti-beta II Tubulin antibody [7B9] (ab28035) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution
Predicted band size: 50 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
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beta II Tubulin was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate with ab28035 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab28035 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/1000 dilution.
Lane 1: Neuro-2a whole cell lysate 10µg.
Lane 2: ab28035 IP in Neuro-2a whole cell lysate.
Lane 3: Mouse monoclonal IgG (ab18443) instead of ab28035 in Neuro-2a whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 seconds.
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All lanes : Anti-beta II Tubulin antibody [7B9] (ab28035) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 2 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution
Predicted band size: 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: lane 1, 2: 1 second
lane 3: 3 seconds -
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a cells labelling beta II Tubulin with ab28035 at 1/50 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cell line. ab179513 anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab28035 at a 1/500 dilution followed by ab150080 at a 1/500 dilution.
Negative control 2: ab179513 at a 1/500 dilution followed by ab150113 at a 1/1000 dilution.
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All lanes : Anti-beta II Tubulin antibody [7B9] (ab28035) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
Lane 2 : Y79 (human retinoblastoma retinoblastoma), whole cell lysate
Lane 3 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 50 kDa
Exposure time: 180 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 cells labelling beta II Tubulin with ab28035 at 1/50 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cell line. ab179513 anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab28035 at a 1/500 dilution followed by ab150080 at a 1/500 dilution.
Negative control 2: ab179513 at a 1/500 dilution followed by ab150113 at a 1/1000 dilution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab28035 は 4 報の論文で使用されています。
- Wang G et al. Human amniotic epithelial cells regulate osteoblast differentiation through the secretion of TGFß1 and microRNA-34a-5p. Int J Mol Med 41:791-799 (2018). PubMed: 29207015
- He X et al. Resveratrol Attenuates Formaldehyde Induced Hyperphosphorylation of Tau Protein and Cytotoxicity in N2a Cells. Front Neurosci 10:598 (2016). PubMed: 28197064
- Narvi E et al. Altered TUBB3 expression contributes to the epothilone response of mitotic cells. Br J Cancer 108:82-90 (2013). PubMed: 23321512
- Moody SA et al. Developmental expression of a neuron-specific beta-tubulin in frog (Xenopus laevis): a marker for growing axons during the embryonic period. J Comp Neurol 364:219-30 (1996). PubMed: 8788246