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AB616

Anti-beta Galactosidase 抗体

Anti-beta Galactosidase antibody

4

(30 Reviews)

|

(128 Publications)

Rabbit Polyclonal GLB1/Beta-galactosidase antibody. Suitable for WB and reacts with Escherichia coli samples. Cited in 128 publications.

別名を表示する

b0344, JW0335, lacZ, Beta-galactosidase, Beta-gal, Lactase

1 Images
Western blot - Anti-beta Galactosidase antibody (AB616)
  • WB

Supplier Data

Western blot - Anti-beta Galactosidase antibody (AB616)

Western blotting using ab616. Lane 1 shows 80 ng and lane 2 shows 20 ng loaded onto gel. Results for non-reducing conditions of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under reducing conditions are shown on the right. The membrane was probed with ab616 at 1/10,000 dilution.

All lanes:

Western blot - Anti-beta Galactosidase antibody (ab616)

Lanes 1 - 2:

Non-reduced purified recombinant beta Galactosidase protein

Lanes 3 - 4:

reduced purified recombinant beta Galactosidase protein

Predicted band size: 116 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Escherichia coli

アプリケーション

WB

applications

特異性

Cross reactivity against Beta Galactosidase from other tissues and species may occur but have not been specifically determined. Very low background staining has been reported in various assays.

Reactivity data

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製品の詳細

Application Note: This antibody shows inconsistent results between batches in ICC/IF, IF, IHC-P, IHC-Fr and IHC-FoFr. We have received several good Abreviews on these applications, however, we are unable to guarantee positive results. If the antibody is used for IHC, please try fixing with methanol and block with gelatine instead with BSA or NGS. After each antibody incubation, please wash 5x with TBS instead of PBS.We would recommend ab4761 or ab12081 as alternatives.

出荷温度及び保存条件

製品の状態
Liquid
精製度
IgG fraction
精製方法
Ion exchange chromatography
精製に関する特記事項
Beta-Galactosidase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
バッファー組成
Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Beta Galactosidase often called "β-gal" "b-galactosidase" or "b-gal" serves as an important enzyme that hydrolyzes β-galactosides into monosaccharides. It breaks down lactose into glucose and galactose which are easier to absorb. Beta-galactosidase expresses in many organisms such as *E. coli* where it is a well-known component of the lac operon. Its molecular weight in *E. coli* is approximately 116 kDa. The enzyme also appears in human tissues but is synthesized and regulated differently compared to bacterial systems.
Biological function summary

This enzyme is involved in the metabolism of galactose a critical aspect of cellular energy production. It can participate in various metabolic pathways converting complex carbohydrates into simpler sugars. In bacteria beta-galactosidase belongs to a protein complex controlled by the lac operon regulating lactose metabolism. In humans the enzyme takes part in lysosomal functions contributing to the degradation of glycoproteins and glycolipids therefore supporting normal cellular processes.

Pathways

Beta-galactosidase plays an important role in the lactose metabolism pathway. In *E. coli* it coheres with the enzymes of the lac system like lactose permease and transacetylase to facilitate lactose utilization. In human biology it participates in the lysosomal degradation pathway. Its function is associated with proteins such as galactosylceramidase which also break down galactolipids demonstrating its interaction in broader metabolic networks.

Deficiencies or malfunctions in beta-galactosidase lead to metabolic conditions like Galactosialidosis and Morquio syndrome Type B. These are lysosomal storage disorders caused by a lack of enzyme activity leading to accumulation of partially degraded macromolecules. The enzyme's impaired function connects with other proteins such as protective protein cathepsin A in the case of Galactosialidosis causing complex clinical symptoms through disrupted protein interactions and pathways.

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

ターゲットの情報

See full target information lacZ

文献 (128)

Recent publications for all applications. Explore the full list and refine your search

Genesis (New York, N.Y. : 2000) 63:e70022 PubMed40631533

2025

Development of a Hepatocyte-Specific Temporal Genetic Mouse Model Using Albumin Promoter-Driven FlpER2 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Wang,Jiale Wang,Yang Liu,Gordon He,Yasmin Jahan-Mihan,Yuxi Wang,Jie Hu,Audrey Li,Faheem Muhammad,Yan Bi,Baoan Ji

Journal of orthopaedic translation 50:211-222 PubMed39895866

2025

The Vitamin D-Sirt1/PGC1α Axis Regulates Bone Metabolism and Counteracts Osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Cuicui Yang,Lulu Chen,Xiaoli Guo,Haijian Sun,Dengshun Miao

Journal of orthopaedic translation 49:107-118 PubMed39430127

2024

The senolytic agent ABT263 ameliorates osteoporosis caused by active vitamin D insufficiency through selective clearance of senescent skeletal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Cuicui Yang,Wanxin Qiao,Qi Xue,David Goltzman,Dengshun Miao,Zhan Dong

American journal of physiology. Gastrointestinal and liver physiology 326:G495-G503 PubMed38469630

2024

Albumin promoter-driven FlpO expression induces efficient genetic recombination in mouse liver.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Zhu,Yan Yang,Dongfeng Feng,Oliver Wang,Jiaxiang Chen,Jiale Wang,Bin Wang,Yang Liu,Brandy H Edenfield,Ashley N Haddock,Ying Wang,Tushar Patel,Yan Bi,Baoan Ji

Aging and disease : PubMed38377021

2024

Inhibiting Glutaminase Exerts Opposite Effects on Ovariectomy-Induced and Age-Related Reductions in Murine Bone Mass.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Guo,Hongjian Zhao,Zijian Dong,Haozhe Cheng,Meipeng Zhu,Zhong Fang

Molecular cell 84:261-276.e18 PubMed38176414

2024

Translation of non-canonical open reading frames as a cancer cell survival mechanism in childhood medulloblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Damon A Hofman,Jorge Ruiz-Orera,Ian Yannuzzi,Rakesh Murugesan,Adam Brown,Karl R Clauser,Alexandra L Condurat,Jip T van Dinter,Sem A G Engels,Amy Goodale,Jasper van der Lugt,Tanaz Abid,Li Wang,Kevin N Zhou,Jayne Vogelzang,Keith L Ligon,Timothy N Phoenix,Jennifer A Roth,David E Root,Norbert Hubner,Todd R Golub,Pratiti Bandopadhayay,Sebastiaan van Heesch,John R Prensner

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 38:427-442 PubMed36625422

2023

Bmi-1 Overexpression Improves Sarcopenia Induced by 1,25(OH) D Deficiency and Downregulates GATA4-Dependent Rela Transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuyi Wang,Jingyu Zhao,Haiyun Chen,Jiawen Zhou,Ao Chen,Jin'ge Zhang,Yue Wang,Zhiyuan Mao,Jiachen Wang,Xuehan Qiu,Yutong Chen,Rong Wang,Yongjie Zhang,Dengshun Miao,Jianliang Jin

International journal of biological sciences 19:610-624 PubMed36632467

2023

1,25-Dihydroxyvitamin D Deficiency Accelerates Aging-related Osteoarthritis via Downregulation of Sirt1 in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Chen,Jiao Zhang,Jie Li,Ran Qin,Na Lu,David Goltzman,Dengshun Miao,Renlei Yang

Disease models & mechanisms 15: PubMed35972154

2022

Patient-associated mutations in Drosophila Alk perturb neuronal differentiation and promote survival.

Applications

Unspecified application

Species

Unspecified reactive species

Kathrin Pfeifer,Georg Wolfstetter,Vimala Anthonydhason,Tafheem Masudi,Badrul Arefin,Mats Bemark,Patricia Mendoza-Garcia,Ruth H Palmer

Scientific reports 11:11968 PubMed34099837

2021

Osteogenic differentiation of human mesenchymal stromal cells and fibroblasts differs depending on tissue origin and replicative senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Vera Grotheer,Nadine Skrynecki,Lisa Oezel,Joachim Windolf,Jan Grassmann
View all publications

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