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Anti-beta Dystroglycan (phospho Y892) 抗体 [M117] (ab29037)

Submit a review Q&A (1)
Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
  • Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)

Key features and details

  • Mouse monoclonal [M117] to beta Dystroglycan (phospho Y892)
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-beta Dystroglycan (phospho Y892) antibody [M117]
    beta Dystroglycan 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [M117] to beta Dystroglycan (phospho Y892)

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide corresponding to Human beta Dystroglycan (phospho Y892) conjugated to keyhole limpet haemocyanin.

  • ポジティブ･コントロール

    • HepG2 cells or HeLa cells treated with pervanadate.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab29037の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
特記事項
追加情報
ELISA: 1/2000.
WB: 1/1000. Membrane was incubated with diluted antibody in 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature. Predicted molecular weight: 97 kDa (Dystroglycan is expressed as a precursor protein that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

ターゲット情報

  • 関連性

    Dystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha dystroglycan and a 43 kDa transmembrane protein, beta dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin. Phosphorylation at tyrosine 892 within the PPxY motif may regulate c Src interactions with beta dystroglycan, as well as inhibit interactions with WW domain proteins. In skeletal muscle, beta dystroglycan is normally localized to the plasma membrane, however phosphorylation of Tyr892 leads to localization of beta dystroglycan to endosomal compartments along with c Src. Thus, phosphorylation at Tyr892 may have important roles in altering the localization of beta dystroglycan during NMJ formation.

  • 細胞内局在

    Type I membrane protein.

  • 参照データベース

    • 別名

      • 156DAG antibody
      • A3a antibody
      • AGRNR antibody
      • Beta DG antibody
      • Beta dystroglycan antibody
      • DAG 1 antibody
      • DAG1 antibody
      • Dystroglycan antibody
      • Dystrophin associated glycoprotein 1 antibody
      see all

    画像

    • Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
      Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
      All lanes : Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)

      Lane 1 : Lysates from HepG2 cells (untreated)
      Lane 2 : Lysates from HepG2 cells treated with pervanadate (1 mM) for 30 min

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 97 kDa
      Observed band size: 43 kDa why is the actual band size different from the predicted?



      Dystroglycan is expressed as a precursor protein (97kDa) that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa.
    • Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
      Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
      Western blot analysis of HepG2 cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). Blots were probed with anti-beta-dystroglycan (lanes 1 & 2) and anti-beta-dystroglycan (Tyr-892) (lanes 3 & 4).

    参考文献 (0)

    ab29037 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab29037 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    I am currently investigating the antibodies against dystroglycan both alpha and beta and have seen that you have a large selection of different clones available. The antibodies with product codes mentioned above state they have not been referenced in papers yet... is this information up to date or are there still no references or evidence that these antibodies work. If this is the case it would be helpful if you could forward any information/evidence that you that these work. I would also be happy to test them if you were able to send them free of charge.

    Thank you for any assistance you can provide,

    Read More
    Answer

    Thank you for your inquiry.

    I can confirm that we do not know of any publication using the above mentioned antibodies. I would like to reassure you that we do guarantee our product for all species and applications stated on the respective datasheet.

    If you would like to use one of our products in a species or application that it has not been previously been tested in, you might be eligible for our testing discount program.

    https://www.abcam.com/collaborationdiscount

    For more information please see below:

    At Abcam, providing quality service is just as important as providing quality products. Our Abpromise® ensures that you can trust our products to perform as stated on the datasheets, or we will offer a replacement, credit, or refund.

    The Abcam Abpromise® guarantee:
    - 100% Scientific and Customer Support for any product you buy from Abcam or one of our authorized distributors.
    - We guarantee our products work in the tested species and applications stated on the datasheet.
    - We will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase.
    - We investigate any quality concerns raised by customers to ensure our catalog contains products that perform to the highest standards.

    Please note these conditions to our Abpromise®:
    - Protocol information must be provided in order for the claim to be validated.
    - Antibodies tested in recombinant samples only are not guaranteed for use on endogenous samples.
    - “Predicted to react” information on the datasheets is provided for reference only and these species are not guaranteed.
    - Fast Track antibodies are covered for ELISA against the immunizing peptide only.

    https://www.abcam.com/abpromise

    I hope this information is helpful, but please do not hesitate to contact us with further questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com