Anti-beta Dystroglycan 抗体 (ab43125)
Key features and details
- Rabbit polyclonal to beta Dystroglycan
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-beta Dystroglycan antibody
beta Dystroglycan 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to beta Dystroglycan -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rabbit, Cow, Dog
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human Dystroglycan precursor.
Immunogen の所有権に関して (Peptide available as ab43750.) -
ポジティブ･コントロール
- WB: Human brain, heart, skeletal muscle, testes (data not shown), kidney (data not shown), colon (data not shown) tissue lysates and HEK293T cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab43125の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
ターゲット情報
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関連性
Dystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha dystroglycan and a 43 kDa transmembrane protein, beta dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin. Phosphorylation at tyrosine 892 within the PPxY motif may regulate c Src interactions with beta dystroglycan, as well as inhibit interactions with WW domain proteins. In skeletal muscle, beta dystroglycan is normally localized to the plasma membrane, however phosphorylation of Tyr892 leads to localization of beta dystroglycan to endosomal compartments along with c Src. Thus, phosphorylation at Tyr892 may have important roles in altering the localization of beta dystroglycan during NMJ formation. -
細胞内局在
Type I membrane protein. -
参照データベース
- Entrez Gene: 281439 Cow
- Entrez Gene: 476623 Dog
- Entrez Gene: 1605 Human
- Entrez Gene: 13138 Mouse
- Entrez Gene: 100009278 Rabbit
- Omim: 128239 Human
- SwissProt: O18738 Cow
- SwissProt: Q9TSZ6 Dog
see all -
別名
- 156DAG antibody
- A3a antibody
- AGRNR antibody
see all
画像
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Western blot - Anti-beta Dystroglycan antibody (ab43125)All lanes : Anti-beta Dystroglycan antibody (ab43125) at 1/500 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : DAG1 knockout HEK293T cell lysate
Lane 3 : Human skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDaLanes 1-3: Merged signal (red and green). Green - ab43125 observed at 43 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab43125 Anti-beta Dystroglycan antibody was shown to specifically react with beta Dystroglycan in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266263 (knockout cell lysate ab257192) was used. Wild-type and beta Dystroglycan knockout samples were subjected to SDS-PAGE. ab43125 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-beta Dystroglycan antibody (ab43125)All lanes : Anti-beta Dystroglycan antibody (ab43125) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab43125 は 6 報の論文で使用されています。
- Wolf DC et al. Sex-specific differences in KCC2 localisation and inhibitory synaptic transmission in the rat hippocampus. Sci Rep 12:3186 (2022). PubMed: 35210456
- Murphy S et al. Proteomic analysis of the sarcolemma-enriched fraction from dystrophic mdx-4cv skeletal muscle. J Proteomics 191:212-227 (2019). PubMed: 29408692
- Trajanovska S et al. Muscle specific kinase protects dystrophic mdx mouse muscles from eccentric contraction-induced loss of force-producing capacity. J Physiol 597:4831-4850 (2019). PubMed: 31340406
- Murphy S et al. Chemical crosslinking analysis of β-dystroglycan in dystrophin-deficient skeletal muscle. HRB Open Res 1:17 (2018). PubMed: 35528858
- Awad PN et al. KCC2 Regulates Dendritic Spine Formation in a Brain-Region Specific and BDNF Dependent Manner. Cereb Cortex 28:4049-4062 (2018). PubMed: 30169756
- Sciandra F et al. Evaluation of the effect of a floxed Neo cassette within the dystroglycan (Dag1) gene. BMC Res Notes 10:601 (2017). PubMed: 29157305