Anti-beta Defensin 1 抗体 [M11-14b-D10] (ab14425)
Key features and details
- Mouse monoclonal [M11-14b-D10] to beta Defensin 1
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-beta Defensin 1 antibody [M11-14b-D10]
beta Defensin 1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [M11-14b-D10] to beta Defensin 1 -
由来種
Mouse -
アプリケーション
適用あり: WB, ELISA, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Baboon, Macaque monkey -
免疫原
Synthetic peptide:
DHYNCVSSGGQCLYSACPIFTKIQGTCYRGKAKCCK
, corresponding to amino acids 1-36 of Human beta 1 Defensin. -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 0.79% Tris HCl -
Concentration information loading...
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精製度
Protein L purified -
ポリ/モノ
モノクローナル -
クローン名
M11-14b-D10 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14425の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Can be blocked with Recombinant human beta Defensin 1 protein (ab50048).
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 2 µg/ml.
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特記事項 |
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WB
Use at an assay dependent concentration. Can be blocked with Recombinant human beta Defensin 1 protein (ab50048). |
ELISA
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 2 µg/ml. |
ターゲット情報
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機能
Has bactericidal activity. -
組織特異性
Plasma. -
配列類似性
Belongs to the beta-defensin family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 1672 Human
- Omim: 602056 Human
- SwissProt: P60022 Human
- Unigene: 32949 Human
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別名
- BD 1 antibody
- BD-1 antibody
- BD1 antibody
see all
画像
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ab14425 (2µg/ml) staining beta defensin in human kidney, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining in the epithelial layers of the loops of Henle, distal tubules and collecting ducts.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab14425 は 10 報の論文で使用されています。
- Wu J et al. Tumor-Infiltrating CD4+ Central Memory T Cells Correlated with Favorable Prognosis in Oral Squamous Cell Carcinoma. J Inflamm Res 15:141-152 (2022). PubMed: 35035226
- Peled A et al. Loss-of-function mutations in caspase recruitment domain-containing protein 14 (CARD14) are associated with a severe variant of atopic dermatitis. J Allergy Clin Immunol 143:173-181.e10 (2019). PubMed: 30248356
- Roth M et al. Pelargonium sidoides radix extract EPs 7630 reduces rhinovirus infection through modulation of viral binding proteins on human bronchial epithelial cells. PLoS One 14:e0210702 (2019). PubMed: 30707726
- Gazi U et al. Tonsillar antimicrobial peptide (AMP) expression profiles of periodic fever, aphthous stomatitis, pharyngitis, cervical adenitis (PFAPA) patients. Int J Pediatr Otorhinolaryngol 110:100-104 (2018). IHC-P ; Human . PubMed: 29859567
- Bonamy C et al. Expression of the human antimicrobial peptide ß-defensin-1 is repressed by the EGFR-ERK-MYC axis in colonic epithelial cells. Sci Rep 8:18043 (2018). PubMed: 30575780
- Roth M et al. Broncho Vaxom (OM-85) modulates rhinovirus docking proteins on human airway epithelial cells via Erk1/2 mitogen activated protein kinase and cAMP. PLoS One 12:e0188010 (2017). PubMed: 29182620
- Qian YJ et al. Cigarette Smoke Modulates NOD1 Signal Pathway and Human ß Defensins Expression in Human Oral Mucosa. Cell Physiol Biochem 36:457-73 (2015). Human . PubMed: 25968832
- Trend S et al. Antimicrobial protein and Peptide concentrations and activity in human breast milk consumed by preterm infants at risk of late-onset neonatal sepsis. PLoS One 10:e0117038 (2015). ELISA . PubMed: 25643281
- Han Q et al. Human beta-defensin-1 suppresses tumor migration and invasion and is an independent predictor for survival of oral squamous cell carcinoma patients. PLoS One 9:e91867 (2014). IHC ; Human . PubMed: 24658581
- Zoega M et al. Proteinase 3 carries small unusual carbohydrates and associates with alpha-defensins. J Proteomics : (2011). PubMed: 22138257