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リコンビナントRabMAb

Anti-beta Amyloid 1-40 抗体 [EPR23712-2] - BSA and Azide free (ab289991)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)
  • Western blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)
  • Dot Blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)
  • Indirect ELISA - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)
  • Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23712-2] to beta Amyloid 1-40 - BSA and Azide free
  • Suitable for: IHC-P, Indirect ELISA, Dot blot, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 PE Unconjugated

製品の概要

  • 製品名

    Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free
    beta Amyloid 1-40 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23712-2] to beta Amyloid 1-40 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, Indirect ELISA, Dot blot, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: BSA-crosslinked beta-Amyloid (Aß40) 1-40 peptide IHC-P: Human Alzheimer's cerebrum tissue.

  • 特記事項

    ab289991 is the carrier-free version of ab254345

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab289991の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Indirect ELISA
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 4, 86 kDa (predicted molecular weight: 86 kDa).
特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Indirect ELISA
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 4, 86 kDa (predicted molecular weight: 86 kDa).

ターゲット情報

  • 機能

    Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons.
    Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu(2+) and Fe(3+) to Cu(+) and Fe(2+), respectively. Beta-amyloid 42 is a more effective reductant than beta-amyloid 40. Beta-amyloid peptides bind to lipoproteins and apolipoproteins E and J in the CSF and to HDL particles in plasma, inhibiting metal-catalyzed oxidation of lipoproteins. Beta-APP42 may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation. Interaction with overexpressed HADH2 leads to oxidative stress and neurotoxicity.
    Appicans elicit adhesion of neural cells to the extracellular matrix and may regulate neurite outgrowth in the brain.
    The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis.
    N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6).

  • 組織特異性

    Expressed in all fetal tissues examined with highest levels in brain, kidney, heart and spleen. Weak expression in liver. In adult brain, highest expression found in the frontal lobe of the cortex and in the anterior perisylvian cortex-opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Weak expression found in the striate, extra-striate and motor cortices. Expressed in cerebrospinal fluid, and plasma. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Appican is expressed in astrocytes.

  • 関連疾患

    Defects in APP are the cause of Alzheimer disease type 1 (AD1) [MIM:104300]. AD1 is a familial early-onset form of Alzheimer disease. It can be associated with cerebral amyloid angiopathy. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
    Defects in APP are the cause of cerebral amyloid angiopathy APP-related (CAA-APP) [MIM:605714]. A hereditary localized amyloidosis due to amyloid-beta A4 peptide(s) deposition in the cerebral vessels. The principal clinical characteristics are recurrent cerebral and cerebellar hemorrhages, recurrent strokes, cerebral ischemia, cerebral infarction, and progressive mental deterioration. Patients develop cerebral hemorrhage because of the severe cerebral amyloid angiopathy. Parenchymal amyloid deposits are rare and largely in the form of pre-amyloid lesions or diffuse plaque-like structures. They are Congo red negative and lack the dense amyloid cores commonly present in Alzheimer disease. Some affected individuals manifest progressive aphasic dementia, leukoencephalopathy, and occipital calcifications.

  • 配列類似性

    Belongs to the APP family.
    Contains 1 BPTI/Kunitz inhibitor domain.

  • ドメイン

    The basolateral sorting signal (BaSS) is required for sorting of membrane proteins to the basolateral surface of epithelial cells.
    The NPXY sequence motif found in many tyrosine-phosphorylated proteins is required for the specific binding of the PID domain. However, additional amino acids either N- or C-terminal to the NPXY motif are often required for complete interaction. The PID domain-containing proteins which bind APP require the YENPTY motif for full interaction. These interactions are independent of phosphorylation on the terminal tyrosine residue. The NPXY site is also involved in clathrin-mediated endocytosis.

  • 翻訳後修飾

    Proteolytically processed under normal cellular conditions. Cleavage either by alpha-secretase, beta-secretase or theta-secretase leads to generation and extracellular release of soluble APP peptides, S-APP-alpha and S-APP-beta, and the retention of corresponding membrane-anchored C-terminal fragments, C80, C83 and C99. Subsequent processing of C80 and C83 by gamma-secretase yields P3 peptides. This is the major secretory pathway and is non-amyloidogenic. Alternatively, presenilin/nicastrin-mediated gamma-secretase processing of C99 releases the amyloid beta proteins, amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42), major components of amyloid plaques, and the cytotoxic C-terminal fragments, gamma-CTF(50), gamma-CTF(57) and gamma-CTF(59).
    Proteolytically cleaved by caspases during neuronal apoptosis. Cleavage at Asp-739 by either caspase-6, -8 or -9 results in the production of the neurotoxic C31 peptide and the increased production of beta-amyloid peptides.
    N- and O-glycosylated. O-linkage of chondroitin sulfate to the L-APP isoforms produces the APP proteoglycan core proteins, the appicans. The chondroitin sulfate chain of appicans contains 4-O-sulfated galactose in the linkage region and chondroitin sulfate E in the repeated disaccharide region.
    Phosphorylation in the C-terminal on tyrosine, threonine and serine residues is neuron-specific. Phosphorylation can affect APP processing, neuronal differentiation and interaction with other proteins. Phosphorylated on Thr-743 in neuronal cells by Cdc5 kinase and Mapk10, in dividing cells by Cdc2 kinase in a cell-cycle dependent manner with maximal levels at the G2/M phase and, in vitro, by GSK-3-beta. The Thr-743 phosphorylated form causes a conformational change which reduces binding of Fe65 family members. Phosphorylation on Tyr-757 is required for SHC binding. Phosphorylated in the extracellular domain by casein kinases on both soluble and membrane-bound APP. This phosphorylation is inhibited by heparin.
    Extracellular binding and reduction of copper, results in a corresponding oxidation of Cys-144 and Cys-158, and the formation of a disulfide bond. In vitro, the APP-Cu(+) complex in the presence of hydrogen peroxide results in an increased production of beta-amyloid-containing peptides.
    Trophic-factor deprivation triggers the cleavage of surface APP by beta-secretase to release sAPP-beta which is further cleaved to release an N-terminal fragment of APP (N-APP).
    Beta-amyloid peptides are degraded by IDE.

  • 細胞内局在

    Membrane. Membrane > clathrin-coated pit. Cell surface protein that rapidly becomes internalized via clathrin-coated pits. During maturation, the immature APP (N-glycosylated in the endoplasmic reticulum) moves to the Golgi complex where complete maturation occurs (O-glycosylated and sulfated). After alpha-secretase cleavage, soluble APP is released into the extracellular space and the C-terminal is internalized to endosomes and lysosomes. Some APP accumulates in secretory transport vesicles leaving the late Golgi compartment and returns to the cell surface. Gamma-CTF(59) peptide is located to both the cytoplasm and nuclei of neurons. It can be translocated to the nucleus through association with APBB1 (Fe65). Beta-APP42 associates with FRPL1 at the cell surface and the complex is then rapidly internalized. APP sorts to the basolateral surface in epithelial cells. During neuronal differentiation, the Thr-743 phosphorylated form is located mainly in growth cones, moderately in neurites and sparingly in the cell body. Casein kinase phosphorylation can occur either at the cell surface or within a post-Golgi compartment.
  • Information by UniProt

  • 参照データベース

    • 別名

      • A4_HUMAN antibody
      • AAA antibody
      • ABETA antibody
      • ABPP antibody
      • AD1 antibody
      • Adap antibody
      • AICD-50 antibody
      • AICD-57 antibody
      • AICD-59 antibody
      • AID(50) antibody
      • AID(57) antibody
      • AID(59) antibody
      • Alzheimer disease amyloid protein antibody
      • Amyloid intracellular domain 50 antibody
      • Amyloid intracellular domain 57 antibody
      • Amyloid intracellular domain 59 antibody
      • APP antibody
      • APPI antibody
      • Beta amyloid protein 40 antibody
      • Beta APP40 antibody
      • Beta-APP40 antibody
      • Beta-APP42 antibody
      • BetaAPP antibody
      • C31 antibody
      • Cerebral vascular amyloid peptide antibody
      • CVAP antibody
      • Gamma-CTF(50) antibody
      • Gamma-CTF(57) antibody
      • Gamma-CTF(59) antibody
      • PN-II antibody
      • PreA4 antibody
      • Protease nexin-II antibody
      • S-APP-alpha antibody
      • S-APP-beta antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)

      This data was developed using ab254345, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human Alzheimer's cerebrum tissue labelling beta-Amyloid 1-40 with ab254345 at 1/5000 dilution, followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Plaque staining on the human Alzheimer's cerebrum (PMID: 10912918). The section was incubated with ab254345 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Western blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)
      Western blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)
      All lanes : Anti-beta Amyloid 1-40 antibody [EPR23712-2] (ab254345) at 1/1000 dilution

      Lane 1 : BSA-crosslinked beta-Amyloid (Aß42) 1-42 peptide 20 ng
      Lane 2 : BSA-crosslinked beta-Amyloid (Aß40) 1-40 peptide 20 ng
      Lane 3 : BSA-crosslinked beta-Amyloid (Aß39) 1-39 peptide 20 ng
      Lane 4 : BSA-crosslinked beta-Amyloid (Aß38) 1-38 peptide 20 ng
      Lane 5 : BSA-crosslinked beta-Amyloid (Aß37) 1-37 peptide 20 ng
      Lane 6 : BSA-crosslinked beta-Amyloid (Aß36) 1-36 peptide 20 ng
      Lane 7 : BSA-crosslinked beta-Amyloid (Aß16) 1-16 peptide 20 ng

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

      Predicted band size: 86 kDa
      Observed band size: 4, 86 kDa why is the actual band size different from the predicted?



      This data was developed using 254345, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      The band over 75 KD is the BSA-crosslinked Aß40 peptide.

      Exposure time: 48 seconds

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)

      This data was developed using ab254345, the same antibody clone in a different buffer formµlation.

      Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling beta-Amyloid 1-40 with ab254345 at 1/5000 (0.107 µg/ml) followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Negative control: no staining on human cerebrum.The section was incubated with ab254345 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Dot Blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)
      Dot Blot - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)

      This data was developed using ab254345, the same antibody clone in a different buffer formulation.

      Dot blot analysis of beta-Amyloid 1-40 using ab254345 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

      The samples are:

      Lane 1: Beta-Amyloid (Aβ42) 1-42 peptide

      Lane 2: Beta-Amyloid (Aβ40) 1-40 peptide

      Lane 3: Beta-Amyloid (Aβ39) 1-39 peptide

      Lane 4: Beta-Amyloid (Aβ38) 1-38 peptide

      Lane 5: Beta-Amyloid (Aβ37) 1-37 peptide

      Lane 6: Beta-Amyloid (Aβ36) 1-36 peptide

      Lane 7: Beta-Amyloid (Aβ16) 1-16 peptide

      Exposure time: 3 minutes

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

    • Indirect ELISA - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)
      Indirect ELISA - Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)

      This data was developed using ab254345, the same antibody clone in a different buffer formulation.

      Indirect ELISA using ab254345 at 0 - 1000 ng/mL.

      Antigen concentration is 1000 ng/mL.

      Secondary antibody is an Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

       

    • Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)
      Anti-beta Amyloid 1-40 antibody [EPR23712-2] - BSA and Azide free (ab289991)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab289991 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab289991 は論文での使用が確認できていません。

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