Anti-beta Amyloid 1-37 抗体 [IBR-9-16-4] (ab264032)
![Western blot - Anti-beta Amyloid 1-37 antibody [IBR-9-16-4] (ab264032)](https://www.abcam.co.jp/ps/products/264/ab264032/Images/ab264032-490112-anti-beta-amyloid-1-37-antibody-ibr-9-16-4-western-blot.jpg "Western blot - Anti-beta Amyloid 1-37 antibody [IBR-9-16-4] (ab264032)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [IBR-9-16-4] to beta Amyloid 1-37
- Suitable for: WB, Dot blot, Indirect ELISA
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-beta Amyloid 1-37 antibody [IBR-9-16-4]
beta Amyloid 1-37 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [IBR-9-16-4] to beta Amyloid 1-37 -
由来種
Rabbit -
アプリケーション
適用あり: WB, Dot blot, Indirect ELISAmore details
適用なし: IHC-Fr or IHC-P -
種交差性
交差種: Human
非交差種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: BSA-crosslinked beta-Amyloid (Aß37) 1-37 peptide. Indirect ELISA: Human beta amyloid 1-37. Dot blot: Beta-Amyloid (Aß39) 1-39 peptide
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特記事項
ab264032 does not react in: IHC-P with human samples; IHC-Fr with mouse tissues.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
IBR-9-16-4 -
アイソタイプ
IgG
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab264032の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 4,86 kDa (predicted molecular weight: 87 kDa).
|
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| Dot blot |
1/1000.
|
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| Indirect ELISA |
Use at an assay dependent concentration.
Use at 62.5 ng/ml |
| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 4,86 kDa (predicted molecular weight: 87 kDa). |
|
Dot blot
1/1000. |
|
Indirect ELISA
Use at an assay dependent concentration. Use at 62.5 ng/ml |
ターゲット情報
- Information by UniProt
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参照データベース
- Entrez Gene: 351 Human
- SwissProt: P05067 Human
- Unigene: 434980 Human
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別名
- A4_HUMAN antibody
- ABPP antibody
- AICD-50 antibody
see all
画像
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Western blot - Anti-beta Amyloid 1-37 antibody [IBR-9-16-4] (ab264032)All lanes : Anti-beta Amyloid 1-37 antibody [IBR-9-16-4] (ab264032) at 1/1000 dilution
Lane 1 : BSA-crosslinked beta-Amyloid (Aß16) 1-16 peptide 20 ng
Lane 2 : BSA-crosslinked beta-Amyloid (Aß36) 1-36 peptide, 20 ng
Lane 3 : BSA-crosslinked beta-Amyloid (Aß37) 1-37 peptide, 20 ng
Lane 4 : BSA-crosslinked beta-Amyloid (Aß38) 1-38 peptide 20 ng
Lane 5 : BSA-crosslinked beta-Amyloid (Aß39) 1-39 peptide 20 ng
Lane 6 : BSA-crosslinked beta-Amyloid (Aß40) 1-40 peptide 20 ng
Lane 7 : BSA-crosslinked beta-Amyloid (Aß42) 1-42 peptide 20 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 87 kDa
Observed band size: 4,86 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
The band over 75 KD is the BSA-crosslinked Aβ40 peptide.
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![Indirect ELISA - Anti-beta Amyloid 1-37 antibody [IBR-9-16-4] (ab264032)](https://www.abcam.co.jp/ps/products/264/ab264032/Images/ab264032-490107-anti-beta--myloid-1-37-antibody-ibr-9-16-4-indirect-elisa-beta-amyloid.jpg)
Indirect ELISA - Anti-beta Amyloid 1-37 antibody [IBR-9-16-4] (ab264032)Indirect ELISA analysis of Human beta amyloid recombinant protein at 100o ng/ml with ab264032 a concentration range 1000-0 ng/ml. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
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![Dot Blot - Anti-beta Amyloid 1-37 antibody [IBR-9-16-4] (ab264032)](https://www.abcam.co.jp/ps/products/264/ab264032/Images/ab264032-490111-anti-beta--myloid-1-37-antibody-ibr-9-16-4-dot-blot-beta-amyloid.jpg)
Dot Blot - Anti-beta Amyloid 1-37 antibody [IBR-9-16-4] (ab264032)Dot blot analysis of beta Amyloid 1-37 using ab234032 at 1:1000 followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:20,000 dilution.
Lane 1: Beta-Amyloid (Aβ42) 1-42 peptide
Lane 2: Beta-Amyloid (Aβ40) 1-40 peptide
Lane 3: Beta-Amyloid (Aβ39) 1-39 peptide
Lane 4: Beta-Amyloid (Aβ38) 1-38 peptide
Lane 5: Beta-Amyloid (Aβ37) 1-37 peptide
Lane 6: Beta-Amyloid (Aβ36) 1-36 peptide Lane 7: Beta-Amyloid (Aβ16) 1-16 peptideExposure time: 3 minutes
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
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![Anti-beta Amyloid 1-37 antibody [IBR-9-16-4] (ab264032)](https://www.abcam.co.jp/ps/products/264/ab264032/Images/ab264032-490106-Benefits-of-Recombinant-antibodies.jpg)
Anti-beta Amyloid 1-37 antibody [IBR-9-16-4] (ab264032)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab264032 は論文での使用が確認できていません。