Name: Anti-beta Actin antibody [SP124] - Cytoskeleton Marker
Brand: RabMAb
SKU: ab115777
Rating: 4 (1 reviews)

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Western blot - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP124] to beta Actin - Cytoskeleton Marker
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Carrier Free Carrier Free Unconjugated

製品名

Anti-beta Actin antibody [SP124] - Cytoskeleton Marker
beta Actin 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [SP124] to beta Actin - Cytoskeleton Marker
由来種

Rabbit
特異性

The immunogen used for this product shares 71% homology with Gamma actin/actin cytoplasmic 2 and 64% homology with cardiac, smooth, and skeletal muscle actin. Cross-reactivity with these proteins has not been confirmed experimentally.
アプリケーション

適用あり: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
種交差性

交差種: Mouse, Rat, Human
交差が予測される動物種: Rabbit, Chicken, Cow, Dog, Pig
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- IHC-P: Human Placenta, Human placenta, Mouse placenta, and Rat placenta tissue; WB: Raji, HeLa, A549, HEK-293, NIH/3T3 and Raw 264.7 cell lystate; Flow Cyt (Intra): HeLa, NIH/3T3, and C6 cells; ICC/IF: NIH/3T3, and C6 cells.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

製品の状態

Liquid
保存方法

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファー

pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
精製度

Protein A/G purified
特記事項（精製）

Purified from TCS by protein A/G.
ポリ/モノ

モノクローナル
クローン名

SP124
アイソタイプ

IgG
研究分野

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab115777の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB	(1)	1/200. Predicted molecular weight: 42 kDa.
IHC-P		1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
ICC/IF		1/50.
Flow Cyt (Intra)		1/200. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

特記事項
WB 1/200. Predicted molecular weight: 42 kDa.
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
ICC/IF 1/50.
Flow Cyt (Intra) 1/200. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

機能

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
関連疾患

Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
配列類似性

Belongs to the actin family.
翻訳後修飾

ISGylated.
細胞内局在

Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Target information above from: UniProt accession P60709 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 396526 Chicken
- Entrez Gene: 280979 Cow
- Entrez Gene: 403580 Dog
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 414396 Pig
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
- Omim: 102630 Human
- SwissProt: P60706 Chicken
- SwissProt: P60712 Cow
- SwissProt: O18840 Dog
- SwissProt: P60709 Human
- SwissProt: P60710 Mouse
- SwissProt: Q6QAQ1 Pig
- SwissProt: P29751 Rabbit
- SwissProt: P60711 Rat
- Unigene: 520640 Human
- Unigene: 708120 Human
- Unigene: 727576 Human
- Unigene: 328431 Mouse
- Unigene: 391967 Mouse
- Unigene: 94978 Rat
see all
別名
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
- ACTB_HUMAN antibody
- Actin beta antibody
- Actin cytoplasmic 1 antibody
- Actin, cytoplasmic 1, N-terminally processed antibody
- Actx antibody
- b actin antibody
- b-actin antibody
- Beta cytoskeletal actin antibody
- Beta-actin antibody
- BRWS1 antibody
- E430023M04Rik antibody
- Melanoma X actin antibody
- MGC128179 antibody
- PS1TP5 binding protein 1 antibody
- PS1TP5BP1 antibody
see all

Western blot - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

All lanes : Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777) at 1/200 dilution

Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 4 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 5 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 6 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Exposure time: 10 seconds

Blocking/Diluting buffer and concentration 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat placenta tissue sections labeling beta Actin with ab115777 at 1/100 dilution. Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with citrate sodium buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse placenta tissue sections labeling beta Actin with ab115777 at 1/200 dilution. Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with citrate sodium buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human placenta tissue sections labeling beta Actin with ab115777 at 1/200 dilution. Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with citrate sodium buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling beta Actin with purified ab115777 at 1/50 dilutioin. Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

Flow Cytometry analysis of C6 (rat glial tumor glial cell) cells labeling beta Actin with purified ab115777 at 1/200 - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) was used as the secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

Staining of Human beta Actin in a Formalin/PFA-fixed paraffin-embedded section of Human Placenta using ab115777 at a dilution of 1/200.
Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling beta Actin with purified ab115777 at 1/50 dilution. Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

Flow Cytometry analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling beta Actin with purified ab115777 at 1/200 - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) was used secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling beta Actin with purified ab115777 at 1/200 - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) was used as the secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
Western blot - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777) at 1/200 dilution + Raji cell lystate

Predicted band size: 42 kDa
Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

Flow cytometric analysis of rabbit anti-beta Actin (SP124) antibody ab115777 (1/200) in HeLa cells (green) compared to negative control of rabbit IgG (blue)
Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

Flow cytometry protocols
Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

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ab115777 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab115777 は 142 報の論文で使用されています。

Karimi Galougahi K et al. β3 adrenergic agonism: A novel pathway which improves right ventricular-pulmonary arterial hemodynamics in pulmonary arterial hypertension. Physiol Rep 11:e15549 (2023). PubMed: 36597221
Liu R et al. Hsa_circ_0001944 enhanced GSPT1 expression via sponging miR-498 to promote proliferation and invasion of gastric cancer. J Clin Lab Anal 37:e24810 (2023). PubMed: 36597856
Lou Y et al. Expression and clinical significance of undifferentiated embryonic cell transcription factor 1 in breast cancer. Transl Cancer Res 12:150-162 (2023). PubMed: 36760370
Azevedo PO et al. Differential requirement of neutralizing antibodies and T cells on protective immunity to SARS-CoV-2 variants of concern. NPJ Vaccines 8:15 (2023). PubMed: 36781862
Fang C et al. URB2 as an important marker for glioma prognosis and immunotherapy. Front Pharmacol 14:1113182 (2023). PubMed: 37033651

View all Publications for this product

レビューと Q&A

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Application

Western blot

Sample

Rat Cell lysate - whole cell (cardiac muscle)

Gel Running Conditions

Non-reduced Denaturing

Loading amount

20 µg

Specification

cardiac muscle

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Nov 17 2021

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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Key features and details

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製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

特記事項（精製）

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

関連疾患

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (142)

レビューと Q&A

Western blot abreview for Anti-beta Actin antibody [SP124] - Cytoskeleton Marker