Anti-beta 3 Adrenergic Receptor 抗体 [EPR25440-151] (BSA and Azide free) (ab300484)
![Western blot - Anti-beta 3 Adrenergic Receptor antibody [EPR25440-151] (BSA and Azide free) (AB300484)](https://www.abcam.co.jp/ps/products/300/ab300484/Images/AB300484-486699-anti-adrb3-antibody-epr25440151-western-blot-human-fat-tissue-lysate-20-ug.jpg "Western blot - Anti-beta 3 Adrenergic Receptor antibody [EPR25440-151] (BSA and Azide free) (AB300484)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25440-151] to beta 3 Adrenergic Receptor - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-beta 3 Adrenergic Receptor antibody [EPR25440-151] (BSA and Azide free)
beta 3 Adrenergic Receptor 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR25440-151] to beta 3 Adrenergic Receptor - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt (Intra),ICC/IF,IHC-P or IP -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Human fat tissue and bladder tissue lysate.
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特記事項
ab300484 is the carrier-free version of ab300483.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR25440-151 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300484の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis. -
組織特異性
Expressed mainly in adipose tissues. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB3 sub-subfamily. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 155 Human
- Omim: 109691 Human
- SwissProt: P13945 Human
- Unigene: 2549 Human
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別名
- ADR B3 antibody
- ADRB 3 antibody
- ADRB3 antibody
see all
画像
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Western blot - Anti-beta 3 Adrenergic Receptor antibody [EPR25440-151] (BSA and Azide free) (AB300484)All lanes : Anti-beta 3 Adrenergic Receptor antibody [EPR25440-151] (ab300483) at 1/1000 dilution
Lane 1 : Human fat tissue lysate at 20 µg
Lane 2 : Human spleen tissue lysate at 40 µg
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilutionThis data was developed using ab300483, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16177222).
Negative control: human spleen (PMID: 11564599)
Exposure time: 15 seconds.
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![Western blot - Anti-beta 3 Adrenergic Receptor antibody [EPR25440-151] (BSA and Azide free) (AB300484)](https://www.abcam.co.jp/ps/products/300/ab300484/Images/AB300484-486700-anti-adrb3-antibody-epr25440151-western-blot-human-bladder-tissue-lysate-20-ug.jpg)
Western blot - Anti-beta 3 Adrenergic Receptor antibody [EPR25440-151] (BSA and Azide free) (AB300484)Anti-beta 3 Adrenergic Receptor antibody [EPR25440-151] (ab300483) at 1/1000 dilution + human bladder tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilutionThis data was developed using ab300483, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16177222).
Exposure time: 10 seconds.
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![Anti-beta 3 Adrenergic Receptor antibody [EPR25440-151] (BSA and Azide free) (AB300484)](https://www.abcam.co.jp/ps/products/300/ab300484/Images/AB300484-3-Benefits-of-Recombinant-antibodies.jpg)
Anti-beta 3 Adrenergic Receptor antibody [EPR25440-151] (BSA and Azide free) (AB300484)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab300484 は論文での使用が確認できていません。
