AB6608

Anti-beta 2 Microglobulin 抗体

Anti-beta 2 Microglobulin antibody

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(4 Publications)

Rabbit Polyclonal beta 2 Microglobulin antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human B2M.

別名を表示する

CDABP0092, HDCMA22P, B2M, Beta-2-microglobulin

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, ICC/IF

applications

免疫原

Native Full Length Protein corresponding to Human B2M.

P61769

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "" } } }

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製品の詳細

The antibody reacts with b2-microglobulin isolated from human urine showing a 12.8 kDa band under non-reducing condition of SDS-PAGE.

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出荷温度及び保存条件

製品の状態

Liquid

精製度

IgG fraction

精製方法

Ion exchange chromatography

精製に関する特記事項

Anti-beta-2-Microglobulin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.

バッファー組成

Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC I) and plays an important role in presenting peptides to the immune system. B2M weighs approximately 11.8 kDa and is found abundantly in all nucleated cells. It has alternate names such as B2 microglobulin or beta-2-microglobulin. This protein is present in the cell membrane as a part of the MHC I which is important for immune surveillance. Additionally B2M is detectable in various biological fluids including serum and its levels can reflect physiological and pathological states.

Biological function summary

Beta-2-microglobulin is important for the stability and transport of MHC class I molecules to the cell surface. As part of the MHC class I complex B2M assists in binding peptides allowing immune cells to identify and target pathogen-infected cells. Without B2M the MHC class I molecules are not properly expressed on the cell surface disrupting immune recognition. In laboratory settings researchers often use anti-beta-2-microglobulin antibodies to investigate its role in MHC class I function.

Pathways

Beta-2-microglobulin interacts significantly with the immune system most notably in the antigen processing and presentation pathway. It works alongside proteins such as the heavy chain of MHC class I. B2M is important in the pathway that involves the transport of antigens to the endoplasmic reticulum where they are loaded onto MHC class I molecules for inspection by cytotoxic T cells. Another related pathway is the tapasin-mediated processing of antigen peptides highlighting the indispensable role of B2M in immune response regulation.

Beta-2-microglobulin is associated with conditions such as beta-2-microglobulin amyloidosis and certain lymphoproliferative disorders. Elevated levels of B2M in serum serve as a marker for diseases like multiple myeloma where the protein level correlates with disease severity. B2M-related amyloidosis frequently occurs in patients undergoing long-term dialysis where amyloid deposits accumulate in tissues. Linking B2M to immune system dysfunction studies have shown interactions with other proteins including components of the immune system like HLA-A and HLA-B highlighting B2M's relevance in diagnosing and understanding these conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed : 25356553).

See full target information B2M

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Immunity 54:1772-1787.e9 PubMed34289378

2021

β-microglobulin triggers NLRP3 inflammasome activation in tumor-associated macrophages to promote multiple myeloma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Hofbauer,Dimitrios Mougiakakos,Luca Broggini,Mario Zaiss,Maike Büttner-Herold,Christian Bach,Bernd Spriewald,Frank Neumann,Savita Bisht,Jens Nolting,Robert Zeiser,Shaima'a Hamarsheh,Martin Eberhardt,Julio Vera,Cristina Visentin,Chiara Maria Giulia De Luca,Fabio Moda,Stefan Haskamp,Cindy Flamann,Martin Böttcher,Katrin Bitterer,Simon Völkl,Andreas Mackensen,Stefano Ricagno,Heiko Bruns

Antioxidants & redox signaling 15:635-44 PubMed21050141

2011

Dimerization of soluble disulfide trap single-chain major histocompatibility complex class I molecules dependent on peptide binding affinity.

Applications

Species

Human

Eleni Kotsiou,Joanna Brzostek,Izabela Lenart,Antony N Antoniou,Julian Dyson,Keith G Gould

Hepatology (Baltimore, Md.) 51:654-9 PubMed20017200

2009

Huh-7: a human "hemochromatotic" cell line.

Applications

ICC/IF

Species

Human

Chiara Vecchi,Giuliana Montosi,Antonello Pietrangelo

PloS one 3:e3858 PubMed19050760

2008

GPRC6A null mice exhibit osteopenia, feminization and metabolic syndrome.

Applications

Species

Human

Min Pi,Ling Chen,Min-Zhao Huang,Wenyu Zhu,Brian Ringhofer,Junming Luo,Lane Christenson,Benyi Li,Jianghong Zhang,P David Jackson,Pieter Faber,Kurt R Brunden,John J Harrington,L Darryl Quarles

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-beta 2 Microglobulin 抗体

Anti-beta 2 Microglobulin antibody

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

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Abcam product promise