Anti-beta 2 Defensin/BD-2 抗体 (ab9871)
Key features and details
- Goat polyclonal to beta 2 Defensin/BD-2
- Suitable for: Sandwich ELISA, Neutralising, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-beta 2 Defensin/BD-2 antibody
beta 2 Defensin/BD-2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to beta 2 Defensin/BD-2 -
由来種
Goat -
アプリケーション
適用あり: Sandwich ELISA, Neutralising, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human beta 2 Defensin/BD-2 aa 1 to the C-terminus. Highly pure (>98%) recombinant Human beta 2 Defensin/hBD-2. Full length mature beta 2 Defensin hBD-2 used as immunogen.
Database link: O15263 -
ポジティブ・コントロール
- recombinant beta 2 Defensin/BD-2 ab9872
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特記事項
hBD-2 is a antimicrobial peptide, which belongs to the distinct family of beta-defensins.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Centrifuge vial prior to opening. Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described, then no further reconstitution is necessary. The lyophilized antibody is stable for at least 2 years from date of receipt at -20C. The reconstituted antibody is stable for at least two weeks at 2-8C. Frozen aliquots are stable for at least 6 months when stored at -20C. Avoid repeated freeze-thaw cycles. -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.20
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
一次抗体 備考
hBD-2 is a antimicrobial peptide, which belongs to the distinct family of beta-defensins. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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ELISA pair antibody
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9871の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Sandwich ELISA |
Use at an assay dependent concentration.
To detect beta 2 Defensin/hBD-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83509 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant beta 2 Defensin/hBD-2. |
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Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of Human beta 2 Defensin/hBD-2 (1.0 ug/ml), a concentration of 5.0 - 8.0 ug/ml of this antibody is required. |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 7 kDa.
To detect beta 2 Defensin/hBD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant beta 2 Defensin/hBD-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions |
特記事項 |
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Sandwich ELISA
Use at an assay dependent concentration. To detect beta 2 Defensin/hBD-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83509 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant beta 2 Defensin/hBD-2. |
Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of Human beta 2 Defensin/hBD-2 (1.0 ug/ml), a concentration of 5.0 - 8.0 ug/ml of this antibody is required. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 7 kDa. To detect beta 2 Defensin/hBD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant beta 2 Defensin/hBD-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions |
ターゲット情報
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機能
Has antibacterial activity. -
組織特異性
Expressed in the skin and respiratory tract. -
配列類似性
Belongs to the beta-defensin family. LAP/TAP subfamily. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 100289462 Human
- Entrez Gene: 1673 Human
- Omim: 602215 Human
- SwissProt: O15263 Human
- Unigene: 105924 Human
- Unigene: 721744 Human
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別名
- BD 2 antibody
- BD-2 antibody
- BD2 antibody
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab9871 は 9 報の論文で使用されています。
- van der Krieken DA et al. Gram-positive anaerobic cocci guard skin homeostasis by regulating host-defense mechanisms. iScience 26:106483 (2023). PubMed: 37096035
- Johnson V et al. Activated Mesenchymal Stromal Cell Therapy for Treatment of Multi-Drug Resistant Bacterial Infections in Dogs. Front Vet Sci 9:925701 (2022). PubMed: 35812842
- Chow L et al. Antibacterial activity of human mesenchymal stem cells mediated directly by constitutively secreted factors and indirectly by activation of innate immune effector cells. Stem Cells Transl Med 9:235-249 (2020). PubMed: 31702119
- Smits JPH et al. Targeting the Cutaneous Microbiota in Atopic Dermatitis by Coal Tar via AHR-Dependent Induction of Antimicrobial Peptides. J Invest Dermatol 140:415-424.e10 (2020). PubMed: 31344386
- Landman S et al. Intradermal injection of low dose human regulatory T cells inhibits skin inflammation in a humanized mouse model. Sci Rep 8:10044 (2018). PubMed: 29968819
- de Koning HD et al. A comprehensive analysis of pattern recognition receptors in normal and inflamed human epidermis: upregulation of dectin-1 in psoriasis. J Invest Dermatol 130:2611-20 (2010). PubMed: 20631729
- Pignatti P et al. Tracheostomy and related host-pathogen interaction are associated with airway inflammation as characterized by tracheal aspirate analysis. Respir Med 103:201-8 (2009). PubMed: 18980836
- Herr C et al. Suppression of pulmonary innate host defence in smokers. Thorax 64:144-9 (2009). PubMed: 18852155
- Fahlgren A et al. Increased expression of antimicrobial peptides and lysozyme in colonic epithelial cells of patients with ulcerative colitis. Clin Exp Immunol 131:90-101 (2003). PubMed: 12519391