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Anti-Beclin 1 抗体 [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)

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Western blot - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)
  • Western blot - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)
  • Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)
  • Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)
  • Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)
  • Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [EPR19662] to Beclin 1 - Chimeric
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric)
    Beclin 1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [EPR19662] to Beclin 1 - Chimeric

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, IPmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, HepG2, HEK-293, MCF7, NIH/3T3, A20, C2C12, C6, PC-12, human liver, mouse heart, mouse spleen, rat heart and rat spleen lysates IP: HeLa, RAW264.7 and PC-12 cells.

  • 特記事項

    Ab289864 does not react with mouse in immunoprecipitation. 

    This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab207612). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab289864の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Predicted molecular weight: 52 kDa.
IP
1/30.
特記事項
WB
1/1000. Predicted molecular weight: 52 kDa.
IP
1/30.

ターゲット情報

  • 機能

    Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.

  • 組織特異性

    Ubiquitous.

  • 配列類似性

    Belongs to the beclin family.

  • 細胞内局在

    Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • APG6 antibody
    • ATG 6 antibody
    • ATG6 antibody
    • ATG6 autophagy related 6 homolog antibody
    • Bcl-2-interacting protein beclin antibody
    • Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody
    • Beclin 1 autophagy related antibody
    • Beclin-1 antibody
    • Beclin1 antibody
    • BECN 1 antibody
    • Becn1 antibody
    • BECN1_HUMAN antibody
    • Coiled coil myosin like BCL2 interacting protein antibody
    • Coiled-coil myosin-like BCL2-interacting protein antibody
    • GT197 antibody
    • Protein GT197 antibody
    • VPS 30 antibody
    • VPS30 antibody
    see all

画像

  • Western blot - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)
    Western blot - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)
    All lanes : Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 3 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
    Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 6 : A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate
    Lane 7 : C2C12 (mouse myoblasts myoblast), whole cell lysate
    Lane 8 : C6 (rat glial tumor glial cell), whole cell lysate
    Lane 9 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-6 & 8-9 : Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1/20000 dilution
    Lane 7 : Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1/20000 dilution (Goat Anti-Mouse IgG Fc (HRP) preadsorbed)

    Predicted band size: 52 kDa
    Observed band size: 52 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST. 

    Exposure time: Lane 1-5, 9: 15 seconds Lane 6-8: 5.5 seconds

  • Western blot - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)
    Western blot - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)
    All lanes : Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864) at 1/1000 dilution

    Lane 1 : Human liver tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse spleen tissue lysate
    Lane 4 : Rat heart tissue lysate
    Lane 5 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1/20000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST. 

    Exposure time: Lane 1: 126 seconds Lane: 2, 4: 3 minutes Lane 3, 5: 15 seconds. 

  • Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)
    Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)

    Beclin 1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate with AB289864 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289864 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 μg

    Lane 2: ab289864 IP in HeLa whole cell lysate

    Lane 3: Mouse monoclonal IgG2a (ab18413) instead of ab289864 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 23 seconds. 

    Observed MW (kDa): 52. 

  • Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)
    Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)

    Beclin 1 was immunoprecipitated from 0.35 mg RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate with ab289864 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289864 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.

    Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate 10 μg

    Lane 2: Ab289864 IP in RAW264.7 whole cell lysate

    Lane 3: Mouse monoclonal IgG2a (ab18413) instead of ab289864 in RAW264.7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 75 seconds. 

    Observed MW (kDa): 52. 

     

  • Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)
    Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)

    Beclin 1 was immunoprecipitated from 0.35 mg PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate with ab289864 at 1/30 dilution (2 μg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab289864 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.

    Lane 1: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate 10 μg

    Lane 2: ab289864 IP in PC-12 whole cell lysate

    Lane 3: Mouse monoclonal IgG2a (ab18413) instead of ab289864 in PC-12 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 75 seconds. 

    Observed MW (kDa): 52. 

     

  • Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)
    Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab289864 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab289864 は論文での使用が確認できていません。

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