Anti-Bcr 抗体 [EP535Y] (ab40779)
![Western blot - Anti-Bcr antibody [EP535Y] (ab40779)](https://www.abcam.co.jp/ps/products/40/ab40779/Images/ab40779-415224-anti-bcr-antibody-ep535y-westernblot-k-562-human.jpg "Western blot - Anti-Bcr antibody [EP535Y] (ab40779)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP535Y] to Bcr
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Bcr antibody [EP535Y]
Bcr 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP535Y] to Bcr -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- K562 cell lysate, human lymphoma, HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP535Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab40779の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/100 - 1/250.
|
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| WB |
1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 143 kDa).
|
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
ICC/IF
1/100 - 1/250. |
|
WB
1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 143 kDa). |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity. -
関連疾患
Note=A chromosomal aberration involving BCR is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). -
配列類似性
Contains 1 C2 domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 Rho-GAP domain. -
ドメイン
The region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.
The DH domain is involved in interaction with CCPG1. -
翻訳後修飾
Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2. - Information by UniProt
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参照データベース
- Entrez Gene: 613 Human
- Omim: 151410 Human
- SwissProt: P11274 Human
- Unigene: 517461 Human
- Unigene: 715409 Human
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別名
- ALL antibody
- bcr antibody
- BCR/ABL FUSION GENE, INCLUDED antibody
see all
画像
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Western blot - Anti-Bcr antibody [EP535Y] (ab40779)Anti-Bcr antibody [EP535Y] (ab40779) at 1/1000 dilution (Purified) + K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 143 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcr antibody [EP535Y] (ab40779)](https://www.abcam.co.jp/ps/products/40/ab40779/Images/ab40779-53949-anti-bcr-antibody-ep535y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcr antibody [EP535Y] (ab40779)ab40779, staining human lymphoma by immunohistochemistry, paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Bcr antibody [EP535Y] (ab40779)](https://www.abcam.co.jp/ps/products/40/ab40779/Images/ab40779-53948-anti-bcr-antibody-ep535y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Bcr antibody [EP535Y] (ab40779)ab40779, staining HeLa cells by immunofluorescence. -
![Anti-Bcr antibody [EP535Y] (ab40779)](https://www.abcam.co.jp/ps/products/40/ab40779/Images/ab40779-5-benefits-of-recombinant-antibodies.png)
Anti-Bcr antibody [EP535Y] (ab40779)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab40779 は 1 報の論文で使用されています。
- Guo Z et al. Proteomics strategy to identify substrates of LNX, a PDZ domain-containing E3 ubiquitin ligase. J Proteome Res 11:4847-62 (2012). PubMed: 22889411